Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern
2008467
 
Ordering Recommendation
Mnemonic
ANA R PAT
Methodology
Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Multiplex Bead Assay/Semi-Quantitative Immunoblot
Performed
Sun-Sat
Reported
1-8 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)  
Storage/Transport Temperature
Refrigerated  
Unacceptable Conditions
Non-serum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0050639Nuclear Antibody (ANA) by IFA, IgG By report
0050215Double-​Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA Effective August 20, 2012
Test Number Components Reference Interval
Double-​Stranded DNA (dsDNA) Antibody, IgG by ELISA None Detected.
2002693 Double-​Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10
2005287Chromatin Antibody, IgG Effective July 18, 2011
19 Units or less Negative
20-​60 Units Moderate Positive
61 Units or greater Strong Positive
0050653Extractable Nuclear Antigen Antibodies (RNP, Smith, Scleroderma, SSA, & SSB)
Test Number Components Reference Interval
0050470RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG Refer to report
Smith (ENA) Antibody, IgG Refer to report
0050691SSA (Ro) (ENA) Antibody, IgG Refer to report
0050692SSB (La) (ENA) Antibody, IgG Refer to report
0050599Scleroderma (Scl-​70) (ENA) Antibody, IgG Refer to report
2001601RNA Polymerase III Antibody, IgG
RNA Polymerase III Antibody, IgG
19 Units or less Negative
20-​39 Units Weak Positive
40-​80 Units Moderate Positive
81 Units or greater Strong Positive
Interpretive Data
Refer to report.
Note
The Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern begins with Nuclear Antibody (ANA) by IFA, IgG. Depending on findings, one or more reflexive tests may be required. Tests added may include Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidiae luciliae); Chromatin Antibody, IgG; Extractable Nuclear Antigen Antibodies (RNP, Smith, Scleroderma, SSA, & SSB); PM/Scl-100 Antibody, IgG, by Immunblot; and/or RNA Polymerase III Antibody, IgG. Additional charges apply.
CPT Code(s)
86039; If homogenous pattern add 86225 and 83516 - if reflexed add 86256; If speckled pattern add 86235 x5; If nucleolar pattern add 86235and 83516 x2
Components
Component Test Code*Component Chart Name
2008516Anti-Nuclear Antibody (ANA) by IFA, IgG
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Antinuclear Antibody
  • FANA
  • Fluorescent Antinuclear Antibody