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Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern
2008467
Ordering Recommendation
For the differential diagnosis of connective tissue diseases.
Mnemonic
ANA R PAT
Methodology
Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Multiplex Bead Assay/Semi-Quantitative Immunoblot
Performed
Sun-Sat
Reported
1-8 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)  
Storage/Transport Temperature
Refrigerated  
Unacceptable Conditions
Non-serum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective May 18, 2015 
 
Test Number Components Reference Interval
 dsDNA (Double Stranded DNA) Antibody, IgG None detected.
Interpretive Data
Refer to report.  
Note
The Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern begins with Nuclear Antibody (ANA) by IFA, IgG. Depending on findings, one or more reflexive tests may be required. Tests added may include Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidiae luciliae); Chromatin Antibody, IgG; RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 (Ro) (ENA) Antibody, IgG; SSA 60 (Ro) (ENA) Antibody, IgG; SSB (La) (ENA) Antibody, IgG; Scleroderma (Scl-70) (ENA) Antibody, IgG; PM/Scl-100 Antibody, IgG, by Immunoblot; and/or RNA Polymerase III Antibody, IgG. Additional charges apply.
CPT Code(s)
86039; if homogenous pattern add 86225 and 83516; if reflexed add 86256; if speckled pattern add 86235 x6; If nucleolar pattern add 86235 x2 and 83516
Components
Component Test Code*Component Chart NameLOINC
2008516Anti-Nuclear Antibody (ANA) by IFA, IgG 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Antinuclear Antibody
  • FANA
  • Fluorescent Antinuclear Antibody