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Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern
2008467
Ordering Recommendation

Initial screen for autoimmune connective tissue diseases.

Mnemonic
ANA R PAT
Methodology
Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot
Performed
Sun-Sat
Reported
1-8 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL) 
Storage/Transport Temperature
Refrigerated 
Unacceptable Conditions
Non-serum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Effective November 14, 2016 
Test Number
Components
Reference Interval
Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA PatternLess than 1:40
2003040PM/Scl-100 Antibody, IgG by ImmunoblotNegative
 Double-Stranded DNA (dsDNA) Ab IgG ELISANone detected.
2002693Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae)Less than 1:10
2005287Chromatin Antibody, IgG19 Units or less: Negative
20-60 Units: Moderate Positive
61 Units or greater: Strong Positive
2001601RNA Polymerase III Antibody, IgG19 Units or less: Negative
20-39 Units: Weak Positive
40-80 Units: Moderate Positive
81 Units or greater: Strong Positive
0050599Scleroderma (Scl-70) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
0050470RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
0050085Smith (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
2012074SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
ComponentsReference Interval
SSA 52 (Ro) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA 60 (Ro) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050692SSB (La) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data
Refer to report.

Note
The Anti-Nuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern begins with Nuclear Antibody (ANA) by IFA, IgG. Depending on findings, one or more reflexive tests may be required. Tests added may include Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidiae luciliae); Chromatin Antibody, IgG; RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 (Ro) (ENA) Antibody, IgG; SSA 60 (Ro) (ENA) Antibody, IgG; SSB (La) (ENA) Antibody, IgG; Scleroderma (Scl-70) (ENA) Antibody, IgG; PM/Scl-100 Antibody, IgG, by Immunoblot; and/or RNA Polymerase III Antibody, IgG. Additional charges apply.
CPT Code(s)
86039; if homogenous pattern add 86225 and 83516; if reflexed add 86256; if speckled pattern add 86235 x6; If nucleolar pattern add 86235 x2 and 83516
Components
Component Test Code*Component Chart NameLOINC
2008516Anti-Nuclear Antibody (ANA) by IFA, IgG56634-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Antinuclear Antibody
  • FANA
  • Fluorescent Antinuclear Antibody