RBC Band 3 Protein Reduction in Hereditary Spherocytosis
2008460
Ordering Recommendation
Use to confirm diagnosis of hereditary spherocytosis when hemolytic anemia and spherocytes are present.
Mnemonic
RBC BAND3
Methodology
Qualitative Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days  
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA) or green (sodium or lithium heparin). Include a Wright stained slide.  
Specimen Preparation
Transport 4 mL whole blood in the original container. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Clotted or hemolyzed specimens. Specimens older than 7 days.  
Remarks
Specimens must be analyzed within 7 days of collection.  
Stability
Ambient: 3 days; Refrigerated: 7 days; Frozen: Unacceptable  
Reference Interval
Normal  
Interpretive Data
This test can be used to confirm the suspected diagnosis of Hereditary Spherocytosis (HS). HS is a common inherited hemolytic anemia characterized by the presence of spherical erythrocytes (spherocytes). HS can be diagnosed based on family history and clinical features, along with clinical laboratory tests including peripheral smear examination, osmotic fragility (OF), ektacytometry or flow cytometry. Band 3 is the most abundant transmembrane protein found in the human RBC. Eosin-5-maleimide (EMA) dye binds to band 3 on intact RBCs and a reduction of fluorescence intensity is seen in hereditary spherocytosis. This test by flow cytometry has been reported to have a sensitivity of 93 percent for diagnosis of HS. Congenital Dyserythropoietic Anemia Type II, Southeast Asian Ovalocytosis and Hereditary Pyropoikilocytosis are rare disorders that may have a positive result in this test.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
 
CPT Code(s)
88184
Components
Component Test Code*Component Chart Name
2008461RBC Band 3 Protein Reduction in HS
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Aliases
  • Hereditary Spherocytosis, EMA, BAND 3, Osmotic Fragility
  • HS hemolytic anema assay