Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA
Ordering Recommendation
Semi-Quantitative Multiplex Bead Assay/Qualitative Indirect Fluorescent Antibody
1-5 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)  
Storage/Transport Temperature
Unacceptable Conditions
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0051000 Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay Negative: 19 AU/mL or less
Equivocal: 20-​25 AU/mL
Positive: 26 AU/mL or greater
0049191 Glomerular Basement Membrane Antibody, IgG (IFA) Negative
Interpretive Data
The anti-GBM IFA methodology has been shown to be less specific than the semi-quantitative tests for circulating antibodies, such as the Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay (ARUP Test Code 0051000). The antibody used in the Multiplex Bead Assay, although directed to human IgG F(ab')2, can bind to light chain sites common to all immunoglobulins, causing the assay to be more sensitive but less specific. The determination of a positive result is based on a diffuse "linear" staining pattern as viewed through a fluorescent microscope. All positive results should be confirmed by semi-quantitative tests and/or renal biopsy.

Compliance Statement D: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
83516; 86255
Component Test Code*Component Chart NameLOINC
0049191GBM Antibody, IgG by IFA29994-1
0051000GBM Ab, IgG by Multiplex Bead Assay44446-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Anti-GBM Antibody-IgG
  • Antiglomerular Basement Membrane Antibody-IgG
  • Basement Membrane Antibody
  • Basement Membrane, Glomerular Antibody
  • GBM Antibody
  • Glomerular Anti-Basement Membrane