Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA
2008403
Ordering Recommendation
Preferred panel for detecting glomerular basement membrane (GBM) antibodies in suspected or established anti-GBM disease. If positive, may be useful for monitoring treatment response.
Mnemonic
GBM-G PAN
Methodology
Semi-Quantitative Multiplex Bead Assay/Qualitative Indirect Fluorescent Antibody
Performed
Mon-Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
- Patient Preparation
- Collect
- Serum separator tube.
- Specimen Preparation
- Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)
- Storage/Transport Temperature
- Refrigerated.
- Unacceptable Conditions
- Remarks
- Stability
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
| Test Number | Components | Reference Interval |
|---|---|---|
| 0051000 | Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay | Negative: 19 AU/mL or less Equivocal: 20-25 AU/mL Positive: 26 AU/mL or greater |
| 0049191 | Glomerular Basement Membrane Antibody, IgG (IFA) | Negative |
Interpretive Data
When present, IgG antibody to glomerular basement membrane (GBM) antigen detected by either indirect fluorescent antibody (IFA) or multiplex bead assay helps support a diagnosis of Goodpasture syndrome. However, the combined result of both assays performed during initial evaluation improves the diagnostic sensitivity for disease. A positive result in one or both assays should be confirmed by renal biopsy.
Compliance Statement D: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Note
CPT Code(s)
83516; 86255
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0049191 | GBM Antibody, IgG by IFA | 29994-1 |
| 0051000 | GBM Ab, IgG by Multiplex Bead Assay | 44446-3 |
Aliases
- Anti-GBM Antibody-IgG
- Antiglomerular Basement Membrane Antibody-IgG
- Basement Membrane Antibody
- Basement Membrane, Glomerular Antibody
- GBM Antibody
- Glomerular Anti-Basement Membrane