Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA
2008403
Ordering Recommendation
Submit With Order
Mnemonic
GBM-G PAN
Methodology
Semi-Quantitative Multiplex Bead Assay/Qualitative Indirect Fluorescent Antibody
Performed
Mon-Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0051000 Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay Negative: 19 AU/mL or less
Equivocal: 20-​25 AU/mL
Positive: 26 AU/mL or greater
0049191 Glomerular Basement Membrane Antibody, IgG (IFA) Negative
Interpretive Data
The anti-GBM IFA methodology has been shown to be less specific than the semi-quantitative tests for circulating antibodies, such as the Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay (ARUP Test Code 0051000). The antibody used in the Multiplex Bead Assay, although directed to human IgG F(ab')2, can bind to light chain sites common to all immunoglobulins, causing the assay to be more sensitive but less specific. The determination of a positive result is based on a diffuse "linear" staining pattern as viewed through a fluorescent microscope. All positive results should be confirmed by semi-quantitative tests and/or renal biopsy.

See Compliance Statement D: www.aruplab.com/CS
Note
CPT Code(s)
83516; 86255
Components
Component Test Code*Component Chart Name
0049191GBM Antibody, IgG by IFA
0051000GBM Ab, IgG by Multiplex Bead Assay
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Anti-GBM Antibody-IgG
  • Antiglomerular Basement Membrane Antibody-IgG
  • Basement Membrane Antibody
  • Basement Membrane, Glomerular Antibody
  • GBM Antibody
  • Glomerular Anti-Basement Membrane