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Chromosome Analysis, Amniotic Fluid, with Reflex to Genomic Microarray
2008367
Ordering Recommendation

Use to test amniotic fluid for the following indications:
Abnormal US findings
Abnormal prenatal screen
Fetal demise
Genomic microarray testing is performed on normal chromosome analysis results.  In cases with a normal karyotype, microarray studies reveal clinically relevant copy number variations (CNV) in ~6% of fetuses with an ultrasound anomaly and ~2% whose indication is advanced maternal age or positive aneuploidy screen.

Mnemonic
AF REFLEX
Methodology
Giemsa Band/Genomic Microarray (Oligo-SNP Array)
Performed
Sun-Sat
Reported
7-14 days
If reflexed: 7-10 additional days required for microarray
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Time SensitiveTime Sensitive
Cytogenetic Test Request Form Recommended (ARUP form #43097)Cytogenetic Test Request Form Recommended (ARUP form #43097)
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
N/A 
Collect
Amniotic fluid. 
Specimen Preparation
Do not freeze or expose to extreme temperatures. Transport 15-30 mL amniotic fluid in a sterile container. 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
N/A 
Remarks
 
Stability
Ambient: 48 hours; Refrigerated: 48 hours Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data


Note
If Chromosome Analysis is "normal" or "no growth,"  then Genomic Microarray testing will be added.   Additional charges apply.

Maternal cell contamination studies recommended if testing reflexes to the fetal microarray. For maternal cell contamination studies if testing reflexes to the fetal microarray, please submit a maternal blood sample and order Microarray Genomic, Maternal Confirm (ARUP test code 2002369) accompanied by a test request form for the mother (this test is performed at no charge as part of the fetal microarray). Maternal blood should be submitted along with the amniotic fluid sample at the time the test ordered. For questions regarding ordering please contact ARUP's genetic counselor at (800) 242-2787 ext. 2141.

The chromosome analysis studies involve culturing of living cells; therefore, turnaround times given represent average times which are subject to multiple variables. After specimen receipt, results are generally available in an average of 12 days with 7-10 additional days required for microarray.

A processing fee will be charged if this procedure is canceled at the client's request, after the test has been set up, or if the specimen integrity is inadequate to allow culture growth

This test must be ordered using Cytogenetic test request form 43097 or through your ARUP interface. Please submit the Patient History for Prenatal Cytogenetics form with the electronic packing list. Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.
CPT Code(s)
88269; 88235; 88291; if reflexed add 81229
Components
Component Test Code*Component Chart NameLOINC
0097601Chromosome Analysis, Amniotic Fluid33773-3
2009476EER Chrom Analysis AF w/Rflx to Array
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Amniotic Fluid, Chromosome Analysis
  • Array CGH, Prenatal
  • comparative genomic hybridization, prenatal
  • Karyotype, Amniotic Fluid
  • prenatal
  • Prenatal array
  • Prenatal Chromosomes, Amniotic Fluid
  • Prenatal Diagnosis