Celiac Disease Reflexive Cascade
Ordering Recommendation
Preferred reflex screening test for celiac disease. Cascade contains all tests, including IgA levels necessary for initial serologic evaluation of celiac disease.
Quantitative Nephelometry/Semi-Quantitative Enzyme-Linked Immunosorbent Assay//Semi-Quantitative Indirect Fluorescent Antibody
2-6 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, grossly icteric or grossly lipemic specimens.  
After separation from cells: Ambient: 8 hours; Refrigerated: 8 days; Frozen: 1 year (if frozen within 24 hours)  
Reference Interval
Test Number Components Reference Interval
0050340Immunoglobulin A
0-​30 days: 1-​7 mg/dL
1 month: 1-​53 mg/dL
2 months: 3-​47 mg/dL
3 months: 5-​46 mg/dL
4 months: 4-​72 mg/dL
5 months: 8-​83 mg/dL
6 months: 8-​67 mg/dL
7-​8 months: 11-​89 mg/dL
9-​11 months: 16-​83 mg/dL
1 year: 14-​105 mg/dL
2 years: 14-​122 mg/dL
3 years: 22-​157 mg/dL
4 years: 25-​152 mg/dL
5-​7 years: 33-​200 mg/dL
8-​9 years: 45-​234 mg/dL
10 years and older: 68-​378 mg/dL
0051689Celiac Disease Dual Antigen Screen 19 Units or less: Negative -​ No significant level of detectable IgA or IgG antibodies against human tissue transglutaminase or gliadin peptide.

20 Units or greater: Positive -​ Presence of IgA and/or IgG antibodies against human tissue transglutaminase and/or gliadin peptide; suggests possibility of certain gluten sensitive enteropathies such as celiac disease and dermatitis herpetiformis.
0051357Deamidated Gliadin Peptide (DGP) Antibody, IgA 19 Units or less: Negative
20-​30 Units: Weak Positive
31 Units or greater: Positive
0051359Deamidated Gliadin Peptide (DGP) Antibody, IgG 19 Units or less: Negative
20-​30 Units: Weak Positive
31 Units or greater: Positive
0097709Tissue Transglutaminase (tTG) Antibody, IgA 19 Units or less: Negative
20-​30 Units: Weak Positive
31 Units or greater: Moderate to Strong Positive
0050736Endomysial Antibody, IgA by IFA Less than 1:10
0056009Tissue Transglutaminase Antibody, IgG < 20 EU: None Detected
20-​30 EU: Weakly Positive
> 30 EU: Positive
Interpretive Data
Refer to report.
The Celiac Disease Reflexive Cascade begins with Immunoglobulin A. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. Tests added may include Tissue Transglutaminase Antibody, IgA; Tissue Transglutaminase Antibody, IgG; Endomysial Antibody, IgA by IFA; Deamidated Gliadin Peptide (DGP) Antibody, IgA; Deamidated Glaidin Peptide (DGP) Antibody, IgG; and/or Celiac Disease Dual Antigen Screen. Refer to the Celiac Testing Algorithm found at http://www.arupconsult.com/Algorithms/CeliacDz.pdf. Additional charges apply.
CPT Code(s)
82784; If IgA less than7.0 mg/dL, add 83516 x2; If IgA greater than or equal to 7.0 mg/dL but is less than the normal age range, add 83516. If reflexed to tTG IgA and Gliadin IgA, add 83516 x 2. If Reflexed to tTG IgG and Gliadin IgG, add 83516 x2.; If IgA greater than or equal to the normal age range, add 83516. If reflexed, add 86256 and 83516.
Component Test Code*Component Chart Name
0050340Immunoglobulin A
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References