Influenza A Virus H1/H3 Subtyping by Real-Time RT-PCR
2007469
 
Ordering Recommendation
Follow-up test for patients with documented influenza A. Not a first-line test for the detection of suspected influenza in most clinical situations. Detects and subtypes the 2 predominant strains of circulating influenza A (H1N1 and H3N2).
Mnemonic
FLUTYPEPCR
Methodology
Qualitative Reverse Transcription Polymerase Chain Reaction
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Respiratory specimen: Bronchoalveolar lavage (BAL), nasal wash, nasopharyngeal swab, or pleural fluid.  
Specimen Preparation
Fluid: Transfer 1 mL respiratory specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Swabs: Place in viral transport media. Place each specimen in an individually sealed bag.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
 
Remarks
Specimen source required.  
Stability
Ambient: 8 hours; Refrigerated: 5 days; Frozen: 2 months  
Reference Interval
Interpretive Data
This test targets the H3 and 2009-H1 hemagglutinin genes. The current circulating Influenza A strains are detected and typed (H1N1 and H3N2), however, other H1 and H3 subtypes may also be detected.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
87502 Influenza A & B
Components
Component Test Code*Component Chart Name
2007470Influenza A Virus Subtyping Source
2007471Influenza A Virus Subtyping by RT-PCR
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Flu H1,H3 PCR
  • Influenza