Rapid Plasma Reagin (RPR) with Reflex to RPR Titer or T. pallidum Antibody by Particle Agglutination
Ordering Recommendation
Order to confirm a reactive treponemal screening test (eg, CIA, EIA) when using the reverse testing algorithm to screen for syphilis. For traditional CDC recommended algorithm, refer to Rapid Plasma Reagin (RPR) with Reflex to Titer and TP-PA Confirmation (0050478).
Semi-Quantitative Charcoal Agglutination/Semi-Quantitative Particle Agglutination
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL) Do not freeze. Also acceptable: Plasma, if testing is completed within 48 hours of collection.  
Storage/Transport Temperature
Unacceptable Conditions
CSF or other body fluids.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0050471Rapid Plasma Reagin (RPR) with Reflex to Titer  
Test Code Components Reference Interval
 Rapid Plasma Reagin (RPR) RPR (+) = Reactive
RPR (-​) = Nonreactive
 Rapid Plasma Reagin (RPR) Titer < 1:1
0050477Treponema pallidum Antibody (FTA-​ABS), Serum, IgG by IFA with Reflex to Treponema pallidum Antibody by TP-​PA  
Test Code Components Reference Interval
 T.Pallidum Ab, IgG (FTA-​ABS) Nonreactive
0050777 Treponema pallidum Antibody by TP-​PA Nonreactive
Interpretive Data
If RPR is reactive, then a titer to endpoint will be added. If RPR is nonreactive, a TP-PA (MHA) confirmation will be added. Additional charges apply.
CPT Code(s)
86592 RPR; if reflexed, add (nonreactive) TP-PA 86780 or (reactive) 86593 RPR titer
Component Test Code*Component Chart NameLOINC
0050472Rapid Plasma Reagin (RPR)20507-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Rapid Plasma Reagin (RPR) with MHA-TP Confirmation
  • Rapid Plasma Reagin (RPR), Reverse Algorithm, Syphilis Reverse Algorithm
  • Rapid Plasma Reagin Reflex to Titer or TPPA
  • RPR (Diagnosis) with Reflex to Titer and Confirmatory Testing
  • RPR Reflex to TPPA or Titer
  • RPR Reverse Algorithm
  • RPR with MHA-TP Confirmation
  • Syphilis Reverse Testing Algorithm
  • Treponema pallidum (Rapid Plasma Reagin) with Reflex to Titer & TP-PA Confirmation