EWSR1 (22q12) Gene Rearrangement by FISH
2007225
 
Ordering Recommendation
Diagnose members of the Ewing sarcoma family of tumors (ESFT); however, this assay does not identify the translocation partner.
Mnemonic
EWSR1 FISH
Methodology
Fluorescence in situ Hybridization
Performed
Varies
Reported
4-12 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 6 unstained 5-micron slides. (Min: 3 slides) Protect paraffin block from excessive heat. Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Frozen specimens. Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.  
Remarks
Include surgical pathology report.  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

See Compliance Statement A: www.aruplab.com/CS
Note
CPT Code(s)
88368 x2
Components
Component Test Code*Component Chart Name
0049335EWSR1 by FISH
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • 22q12
  • EWSR1 FISH
  • EWSR1 Gene rearrangement