Hepatitis C Virus High-Resolution Genotype by Sequencing
2006898
Ordering Recommendation
Do not order prior to molecular confirmation of positive hepatitis C virus (HCV) screen. Order before initiating HCV therapy to aid in determining therapy of choice, likelihood of response, and probable therapeutic duration. This assay should be used for prognosis and treatment selection when a higher level of subtype resolution is required (ie, non 6a/b vs. type 1 and type 1a vs. 1b).
Submit With Order
Mnemonic
HCV CORE
Methodology
Polymerase Chain Reaction/Sequencing
Performed
Sun-Sat
Reported
5-8 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), plasma preparation tube, or serum separator tube (SST).  
Specimen Preparation
Separate serum or plasma from cells within 6 hours. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Heparinized specimens.  
Remarks
Please submit most recent viral load and test date if available.  
Stability
On cells: Ambient: 6 hours. After separation from cells: Refrigerated: 72 hours; Frozen: 4 months  
Reference Interval
By report
Interpretive Data
Hepatitis C viral RNA is assayed using reverse transcription polymerase chain reaction (RT-PCR) to amplify specific portions of both the Core and NS5B regions of the viral genome. The amplified nucleic acid is sequenced bi-directionally using dye-terminator chemistry (ABI). Sequencing data is compared to a database of characterized sequences.

Isolates of hepatitis C virus are grouped into six major genotypes (1-6). These genotypes are subtyped according to sequence characteristics. Sequencing both the Core and NS5B regions allows for subtyping of all confirmed and most provisional genotypes, including differentiation of 1a from 1b and typing of genotype 6.

This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc.

See Compliance Statement B: www.aruplab.com/CS
Note
This test may be unsuccessful if the HCV RNA viral load is less than log 3.4 or 2500 IU/mL.
CPT Code(s)
87902
Components
Component Test Code*Component Chart Name
2006899Hepatitis C High-Res Genotype by Seq
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • HCV Genotyping
  • HCV Core and NS5B Sequencing
  • HCV Core Sequencing
  • HCV Genotype
  • HCV NS5B Sequencing
  • HCV prognosis
  • HCV Sequencing
  • HCV, Subtype (Hepatitis C Virus Genotyping)