Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1 Antibody Confirmation by Western Blot
2006526
 
Ordering Recommendation
Mnemonic
HIV AGAB
Methodology
Qualitative Enzyme-Linked Immunosorbent Assay/Qualitative Western Blot
Performed
Mon, Wed, Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), green (sodium or lithium heparin), or red (clot activator)  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. Remove particulate material. (Min: 0.5 mL).  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.  
Remarks
 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-​1/O/2) by ELISA Negative
0020284 Human Immunodeficiency Virus Type 1 (HIV-​1) Antibody Confirmation by Western Blot Negative
Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).
Note
This fourth-generation screen test is for the simultaneous qualitative detection of Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2), Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody.

If the HIV-1,2 Combo Antigen/Antibodies screen is repeatedly reactive, then the Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.

Performed and Reported times indicated are for the screening portion of this test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (ARUP test code 0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.
CPT Code(s)
87389; if reflexed, add 86689
Components
Component Test Code*Component Chart Name
2006611HIV-1,2 Combo Antigen/Antibody
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • 4th generation HIV screen; HIV Ag/Ab combination screen
  • Fourth generation HIV
  • HIV 1,2 Combo Antigen/Antibody
  • HIV Diagnosis
  • HIV-1,2 Combo Antigen/Antibody