Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1 Antibody Confirmation by Western Blot
2006526
Ordering Recommendation
Mnemonic
HIV AGAB
Methodology
Qualitative Enzyme-Linked Immunosorbent Assay/Qualitative Western Blot
Performed
Mon, Wed-Sat
Reported
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
- Patient Preparation
- Collect
- Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), green (sodium or lithium heparin), or red (clot activator)
- Specimen Preparation
- Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. Remove particulate material. (Min: 0.5 mL).
- Storage/Transport Temperature
- Refrigerated.
- Unacceptable Conditions
- Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.
- Remarks
- Stability
- After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)
Reference Interval
| Test Number | Components | Reference Interval |
|---|---|---|
| Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA | Negative | |
| 0020284 | Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot | Negative |
Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).
Note
This fourth-generation screen test is for the simultaneous qualitative detection of Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2), Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody.
If the HIV-1,2 Combo Antigen/Antibodies screen is repeatedly reactive, then the Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.
Performed and Reported times indicated are for the screening portion of this test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (ARUP test code 0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.
If the HIV-1,2 Combo Antigen/Antibodies screen is repeatedly reactive, then the Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.
Performed and Reported times indicated are for the screening portion of this test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (ARUP test code 0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.
CPT Code(s)
87389; if reflexed, add 86689
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2006611 | HIV-1,2 Combo Antigen/Antibody | 56888-1 |
Aliases
- 4th generation HIV screen; HIV Ag/Ab combination screen
- Fourth generation HIV
- HIV 1,2 Combo Antigen/Antibody
- HIV Diagnosis
- HIV-1,2 Combo Antigen/Antibody