Not recommended for well-differentiated liposarcomas lacking atypical cells. Immunohistochemical sensitivity, in this setting, is very low and may yield false-negative results. In these cases, MDM2 Gene Amplification by FISH (2003016) is recommended. Refer to ARUP Immunohistochemistry Stain Offerings brochure at www.aruplab.com/ap/resources.
- Patient Preparation
- Tissue or cells.
- Specimen Preparation
- Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 2 slides). If sending precut slides, do not oven bake.
- Storage/Transport Temperature
- Room temperature or refrigerated. Ship in cooled container during summer months.
- Unacceptable Conditions
- Specimens submitted with non-representative tissue type. Depleted specimens.
- IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) and include an ARUP client number. For additional technical details, contact ARUP Client Services at (800) 522-2787.
- Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
|Component Test Code*||Component Chart Name||LOINC|
|2005849||MDM2 IHC Reference Number|
|2005850||MDM2 IHC Result|
- MDM2 IHC: MDM2 immunostaining