WT1 Mutation Detection by Sequencing
2005766
 
Ordering Recommendation
WT1 testing is appropriate for detecting mutations in exons 7 and 9 as well as for the presence of SNP rs16754 in cases of cytogenetically normal AML.
Mnemonic
WT1
Methodology
Polymerase Chain Reaction/Sequencing
Performed
Varies
Reported
12-14 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) OR bone marrow (EDTA).  
Specimen Preparation
Transport 5 mL whole blood (Min: 1 mL) OR 3 mL bone marrow (Min: 1 mL).  
Storage/Transport Temperature
Refrigerated  
Unacceptable Conditions
Serum or plasma. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or severely hemolyzed specimens  
Remarks
 
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
81479
Components
Component Test Code*Component Chart Name
2005767WT1 Mutation Results
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • CN-AML prognostication
  • rs16754
  • WT1 exons 7, 9, SNP rs16754