Japanese Encephalitis Virus Antibodies, IgG and IgM by ELISA
Ordering Recommendation
Use to detect presence of Japanese encephalitis-related IgG and IgM antibodies in individuals with evidence of acute encephalitis syndrome (AES) who have recently traveled to or resided in an endemic country in Asia or the western Pacific. Patient's travel history is necessary to aid in test interpretation. Testing should also be considered for other arthropod-borne viruses with similar symptomology based on clinical presentation and travel history.
New York DOH Approval Status
Specimen Required
Serum separator tube
Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
Refrigerated
Contaminated or heat-inactivated specimens
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 6 months (avoid repeated freeze/thaw cycles)
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Thu
Reported
1-8 days
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
Japanese Encephalitis Virus Antibody IgM | 6.0 IV or less | |
Japanese Encephalitis Virus Antibody IgG | 5.0 IV or less |
Interpretive Data
Patients in the early stage of JEV infection may not have a detectable level of IgG antibody; IgG response may take several days to several weeks to develop. In the absence of detectable IgG antibody, testing for IgM-class antibody is strongly recommended. A positive result (5.1 IV or greater) indicates the presence of IgG antibody to a Flavivirus in the Japanese encephalitis serogroup or the dengue virus serogroup. Cross-species plaque reduction neutralization tests on paired acute and convalescent sera are an acceptable means of determining the Flavivirus causing the antibody production.
A positive result (6.1 IV or greater) indicates the presence of IgM antibody to a flavivirus in the Japanese encephalitis serogroup or the dengue virus serogroup. The best evidence of current infection is a significant change on two appropriately timed specimens with both tests performed in the same laboratory at the same time.
Component |
Unit Of Measure |
Interpretation |
---|---|---|
Japanese Encephalitis Virus Antibody IgG | 1.9 IV or less 2.0 - 5.0 IV 5.1 IV or greater |
Negative - No significant level of detectable Japanese encephalitis virus (JEV) IgG antibody. Equivocal - Questionable presence of JEV IgG antibody. Repeat testing in 10-14 days may be helpful. Positive - JEV IgG antibody detected, which may indicate a current or past infection. |
Japanese Encephalitis Virus Antibody IgM | 3.9 IV or less 4.0 -6.0 IV 6.1 IV or greater |
Negative - No significant level of detectable Japanese encephalitis virus (JEV) IgM antibody. Equivocal - Questionable presence of JEV IgM antibody. Repeat testing in 10-14 days may be helpful. Positive - JEV IgM antibody detected, which may indicate a current or recent infection. A low IgM antibody level may occasionally persist for more than 12 months post-infection. |
Laboratory Developed Test (LDT)
Note
Hotline History
CPT Codes
86790 x2
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2005686 | Japanese Encephalitis Virus Antibody IgM | 63562-3 |
2005688 | Japanese Encephalitis Virus Antibody IgG | 7934-3 |
Aliases
- JE Virus Antibodies
- JE Virus IgG, IgM