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MPL Mutation Detection by Capillary Electrophoresis
2005545
Ordering Recommendation

May be useful when essential thrombocythemia or idiopathic myelofibrosis is suspected in JAK2 V617F-negative individuals.

Mnemonic
MPL
Methodology
Polymerase Chain Reaction/Capillary Electrophoresis
Performed
DNA isolation: Sun-Sat
Assay: Varies
Reported
7-12 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab. 
Specimen Preparation
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens. 
Remarks
 
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely 
Reference Interval
Interpretive Data
Refer to report

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
The test will detect MPL mutations W515K, W515L, W515A, and S505N.
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
2005546MPL Results62948-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • JAK-2 negative MPN diagnosis
  • JAK2 V617 negative testing
  • MPL 1p34