MPL codon 515 Mutation Detection by Pyrosequencing, Quantitative
2005545
 
Ordering Recommendation
May be useful when suspicion for myeloproliferative neoplasms (MPN) is high in JAK2 V617F-negative individuals.
Mnemonic
MPL
Methodology
Polymerase Chain Reaction/Quantitative Pyrosequencing
Performed
DNA isolation: Sun-Sat
Assay:
Varies
Reported
7-12 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA).  
Specimen Preparation
Transport 5 mL whole blood or bone marrow. (Min: 1 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma or serum. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.  
Remarks
 
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report

See Compliance Statement B: www.aruplab.com/CS
Note
The test will detect and quantify MPL codon 515 mutation, including W515K and W515L.
CPT Code(s)
81402
Components
Component Test Code*Component Chart Name
2005546MPL codon 515 results
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • JAK-2 negative MPN diagnosis
  • JAK2 V617 negative testing
  • MPL 1p34