High-Specificity Antiphospholipid Antibodies, IgG and IgM
Ordering Recommendation
Acceptable initial test for strong suspicion of antiphospholipid syndrome (APS).
Submit With Order
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Sun, Tue, Thu
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, grossly hemolyzed, grossly icteric, or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Available Separately Components Reference Interval
No High-​SpecificityAntiphospholipid Antibody, IgG 14 GPL or less: Negative
15-​26 GPL: Indeterminate -​ Repeat testing in 12 weeks
27 GPL or greater: Positive
No High-​SpecificityAntiphospholipid Antibody, IgM 14 MPL or less: Negative
15-​37 MPL: Indeterminate -​ Repeat testing in 12 weeks
38 MPL or greater: Positive
Interpretive Data
High-specificity antiphospholipid IgG and IgM antibodies are directed against a mixture of phosphatidylserine, phosphatidic acid, and beta-2 glycoprotein 1 antigens. These antibodies are more specific than cardiolipin IgG and IgM antibodies in the diagnosis of antiphospholipid syndrome (APS).
GPL = IgG phospholipid units; MPL = IgM phospholipid units
CPT Code(s)
83516 x 2
Component Test Code*Component Chart Name
2005458High-Specificity Antiphospholipid Ab IgG
2005459High-Specificity Antiphospholipid Ab IgM
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • APS-HS