High-Specificity Antiphospholipid Antibodies, IgG and IgM
2005457
Ordering Recommendation
Acceptable initial test for strong suspicion of antiphospholipid syndrome (APS).
Mnemonic
HS APL
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Sun, Tue, Thu
Reported
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Contaminated, heat-inactivated, grossly hemolyzed, grossly icteric, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
 
 
Available Separately Components Reference Interval
No High-​SpecificityAntiphospholipid Antibody, IgG 14 GPL or less: Negative
15-​26 GPL: Indeterminate -​ Repeat testing in 12 weeks
27 GPL or greater: Positive
No High-​SpecificityAntiphospholipid Antibody, IgM 14 MPL or less: Negative
15-​37 MPL: Indeterminate -​ Repeat testing in 12 weeks
38 MPL or greater: Positive
Interpretive Data
High-specificity antiphospholipid IgG and IgM antibodies are directed against a mixture of phosphatidylserine, phosphatidic acid, and beta-2 glycoprotein 1 antigens. These antibodies are more specific than cardiolipin IgG and IgM antibodies in the diagnosis of antiphospholipid syndrome (APS).  
Note
GPL = IgG phospholipid units; MPL = IgM phospholipid units
CPT Code(s)
83516 x 2
Components
Component Test Code*Component Chart Name
2005458High-Specificity Antiphospholipid Ab IgG
2005459High-Specificity Antiphospholipid Ab IgM
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • APS-HS