Urticaria-Inducing Activity
2005413
 
Ordering Recommendation
Determine histamine release in suspected chronic urticaria if urticaria is felt to be due to autoimmune antibodies to the basophil IgE receptor or to IgE.
Mnemonic
UIA
Methodology
Semi-Quantitative Ex Vivo Challenge/Cell Culture/Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Mon, Fri
Reported
2-6 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
Patients taking calcineurin inhibitors should stop their medication for 72 hours prior to draw. Patients on prednisone should be off medication for 2 weeks prior to draw.  
Collect
Plain red.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.5 mL)  
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Specimens other than serum. Contaminated, grossly hemolyzed, or lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective November 14, 2011

10 Units or less
Interpretive Data
A value of greater than 10 Units suggests the presence of basophil stimulating antibodies (or other serum factors).

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note
1) Chronic urticaria (CU) is a common and complex dermatological condition that is suspected when patients experience persistent hives for over 6 weeks. No published evidence of an exogenous allergen as the cause of this disorder exists. About 45 percent of cases have autoantibodies directed against either basophil or mast cell-associated IgE or the high affinity IgE-Fc receptor (Fc epsilon R1 alpha) (Clin Exp Allergy 2009; 39: 777-87).

2) The presence of histamine releasing factors (including but not limited to IgE and Fc epsilon R1 alpha-specific autoantibodies) in the patient serum can be indirectly determined by evaluating basophil/mast cell activation status using histamine release assays, autologous serum-skin test, and flow cytometric measurement of the basophil and mast cell-specific marker CD203c. Serum from CU patients can activate donor basophils, which induces histamine release and CD203c upregulation (J Allergy Clin Immunol 2006; 117: 1430-4).
CPT Code(s)
86352
Components
Component Test Code*Component Chart Name
2005414Urticaria-Inducing Activity
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • CD203c upregulation