Human Immunodeficiency Virus Types 1 and 2 (HIV-1, HIV-2) Antibodies by CIA with Reflex to HIV-1 Antibody Confirmation by Western Blot
Ordering Recommendation
Qualitative Chemiluminescent Immunoassay/Qualitative Western Blot
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube. Also acceptable: Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Heat-inactivated, severely hemolyzed, or lipemic specimens.  
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective November 18, 2013
Test Number Components Reference Interval
 Human Immunodeficiency Virus Types 1 and 2 (HIV-​1, HIV-​2) Antibodies Negative
0020284Human Immunodeficiency Virus Type 1 (HIV-​1) Antibody Confirmation by Western Blot Negative
Interpretive Data
This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).  
If HIV-1 and HIV-2 screen is repeatedly reactive, then Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.

Performed and Reported times indicated are for the screening portion of this reflex test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.
CPT Code(s)
86703; if reflexed, add 86689
Component Test Code*Component Chart NameLOINC
2005378HIV-1,2 Antibodies by CIA42768-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 3rd generation HIV qualitative screening test
  • HIV 1, 2 Combo Antibodies
  • HIV total abs screening
  • HIV1,2 reflex to western blot confirmation