BCR-ABL1, Qualitative with Reflex to BCR-ABL1 Quantitative
2005010
 
Ordering Recommendation
For use in detecting the presence of a BCR-ABL1 fusion, for determining breakpoint status, and for baseline quantitation.
Mnemonic
BCR RFLX
Methodology
Reverse Transcription Polymerase Chain Reaction
Performed
RNA isolation: Sun-Sat
Assay:
Sun, Tue-Fri
Reported
7-10 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or bone marrow (EDTA).  
Specimen Preparation
Transport 5 mL whole blood or 3 mL bone marrow. (Min: 1 mL whole blood or 1 mL bone marrow) Specimens must be received within 48 hours of collection due to lability of RNA.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Serum or plasma. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed specimens. Frozen or clotted specimens.  
Remarks
 
Stability
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note
This reflex assay is recommended when the BCR-ABL1 fusion form is not known or unclear. This reflex assay detects the presence of either the p210 (major breakpoint) or p190 (minor breakpoint). If the presence of either the p210 or p190 BCR-ABL1 fusion is detected, then the appropriate quantitative test will be performed.

If the fusion form is known, refer to BCR-ABL1, Major (p210), Quantitative (ARUP test code 2005017) or BCR-ABL1, Minor (p190), Quantitative (ARUP test code 2005016).
CPT Code(s)
81206; 81207; If reflexed, add 81206 or 81207
If reflexed, add 81206 or 81207
Components
Component Test Code*Component Chart Name
0055553BCR-ABL1, t(9;22) Qual by RT-PCR
2006592BCR-ABL1 Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • p190
  • p210
  • PH1
  • philadelphia chormosome
  • Philadelphia MS
  • t(9;22)