Paroxysmal Nocturnal Hemoglobinuria, WBC
Ordering Recommendation
Use to quantify or monitor paroxysmal nocturnal hemoglobinuria clone size. Preferred initial diagnostic test is Paroxysmal Nocturnal Hemoglobinuria (PNH), RBC and WBC (2005006).
Quantitative Flow Cytometry
1-4 days  
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Specimen Required
Patient Preparation
New York State Clients: Testing is only approved for the Paroxysmal Nocturnal Hemoglobinuria Panel (ARUP test code 2005006) on whole blood specimens.  
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).  
Specimen Preparation
Transport 4 mL whole blood. (Min: 4 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Clotted, hemolyzed or frozen specimens. Bone marrow specimens. Specimens older than 72 hours.
New York State Clients: Specimens for the PNH PAN older than 24 hours ambient or older than 48 hours refrigerated. Clotted, hemolyzed or frozen specimens. Bone marrow specimens.  
Specimens must be analyzed within 72 hours of collection.
New York State Clients: Only the whole blood PNH PAN is acceptable and must be analyzed within 24 hours of collection if ambient or 48 hours of collection if refrigerated.  
Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: PNH PAN-Ambient: 24 hours; Refrigerated: 48 hours. Frozen: Unacceptable  
Reference Interval
By report  
Interpretive Data
This test was developed according to recently published guidelines (Cytometry B Clin Cytom. 2010 Jul; 78 (4):211-30),and includes diagnostic WBC analysis using FLAER, CD14, and CD24 as GPI-linked markers with CD15 and CD33 as lineage-specific markers. The lower limit of detection for this test is 0.1 percent PNH cells and is the most accurate measurement of the PNH clone size used to assist with therapeutic decisions in conventional PNH.

For initial diagnosis of PNH, Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (ARUP test code 2005006) is available. This panel analyzes both RBCs and WBCs.

For delineation of RBC populations Types II and III when the PNH clone is 1 percent or greater, Paroxysmal Nocturnal Hemoglobinuria, High Sensitivity, RBC (ARUP test code 2004366) is available. This test also detects minor (0.005-0.999 percent) PNH clone populations found in bone marrow failure disorders.

Compliance Statement A: Analyte specific reagents (ASR) are used in many laboratory tests necessary for standard medical care and generally do not require U.S. Food and Drug Administration (FDA) approval or clearance. This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. This test should not be regarded as investigational or for research use.
CPT Code(s)
86356 x5
Component Test Code*Component Chart NameLOINC
2005004% PNH Monocytes60554-3
2005005% PNH PMN53831-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
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