Paroxysmal Nocturnal Hemoglobinuria, WBC
2005003
 
Ordering Recommendation
Use to quantify or monitor paroxysmal nocturnal hemoglobinuria clone size. Preferred initial diagnostic test is Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (2005006).
Mnemonic
PNH WBC
Methodology
Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
New York State Clients: Testing is only approved for the Paroxysmal Nocturnal Hemoglobinuria Panel (ARUP test code 2005006) on whole blood specimens.  
Collect
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).  
Specimen Preparation
Transport 4 mL whole blood. (Min: 4 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Clotted, hemolyzed or frozen specimens. Bone marrow specimens. Specimens older than 72 hours.
New York State Clients: Specimens for the PNH PAN older than 24 hours ambient or older than 48 hours refrigerated. Clotted, hemolyzed or frozen specimens. Bone marrow specimens.  
Remarks
Specimens must be analyzed within 72 hours of collection.
New York State Clients: Only the whole blood PNH PAN is acceptable and must be analyzed within 24 hours of collection if ambient or 48 hours of collection if refrigerated.  
Stability
Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: PNH PAN-Ambient: 24 hours; Refrigerated: 48 hours. Frozen: Unacceptable  
Reference Interval
By report
Interpretive Data
This test was developed according to recently published guidelines (Cytometry B Clin Cytom. 2010 Jul; 78 (4):211-30),and includes diagnostic WBC analysis using FLAER, CD14, and CD24 as GPI-linked markers with CD15 and CD33 as lineage-specific markers. The lower limit of detection for this test is 0.1 percent PNH cells and is the most accurate measurement of the PNH clone size used to assist with therapeutic decisions in conventional PNH.

For initial diagnosis of PNH, Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (ARUP test code 2005006) is available. This panel analyzes both RBCs and WBCs.

For delineation of RBC populations Types II and III when the PNH clone is 1 percent or greater, Paroxysmal Nocturnal Hemoglobinuria, High Sensitivity, RBC (ARUP test code 2004366) is available. This test also detects minor (0.005-0.999 percent) PNH clone populations found in bone marrow failure disorders.

See Compliance Statement A: www.aruplab.com/CS
Note
CPT Code(s)
86356 x5
Components
Component Test Code*Component Chart Name
2005004% PNH Monocytes
2005005% PNH PMN
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • PI-Linked Antigens, Monocytes and Granulocytes (Paroxysmal Nocturnal Hemoglobinuria, WBC)