Bordetella pertussis DFA
2004667
Ordering Recommendation
Not recommended for the diagnosis of pertussis due to low sensitivity. CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Submit With Order
Mnemonic
PERTFA
Methodology
Direct Fluorescent Antibody Stain
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Nasal aspirate or washing or nasopharyngeal swab.  
Specimen Preparation
Aspirate or Washing: Transfer to a sterile container.(Min: 0.5 mL)
Nasopharyngeal Swab:
Place swab in Regan-Lowe transport media and incubate for 24-48 hours at 35°C (ARUP supply# 24962). Available online
through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Also acceptable: Swab in Jones Kendrick media or Aimes
with charcoal media, or swabs in described media sent directly without incubation.
If both culture and DFA are ordered, two swabs or swab plus slides are required.  
Storage/Transport Temperature
Room temperature or refrigerated.  
Unacceptable Conditions
Sputum. Swabs or media other than listed above.  
Remarks
Source of specimen is preferred.  
Stability
Incubated Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable.
Unincubated Regan-Lowe, Aimes with charcoal swab, Aspirate or Washing:
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable.
Slides:
Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 month  
Reference Interval
Negative
Interpretive Data
DFA is not recommended for diagnosing infections caused by B. pertussis. Refer instead to Bordetella pertussis Culture (ARUP test code 0060117), or Bordetella pertussis/parapertussis by PCR (ARUP test code 0065080) for amplified DNA testing of respiratory specimens.
Note
CPT Code(s)
87265
Components
Component Test Code*Component Chart Name
2004668B. pertussis DFA Source
2004669B. pertussis DFA Result
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • B. Pertussis Direct Detection
  • B. Pertussis Direct Detection, DFA
  • Pertussis Smear