- Patient Preparation
- New York State Clients: Testing is only approved for the Paroxysmal Nocturnal Hemoglobinuria Panel (ARUP test code 2005006) on whole blood specimens.
- Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).
- Specimen Preparation
- Transport 4 mL whole blood. (Min: 0.5 mL)
- Storage/Transport Temperature
- Unacceptable Conditions
- Clotted, hemolyzed, or frozen specimens. Bone marrow specimens. Specimens older than 4 days.
New York State Clients: Specimens for the PNH PAN older than 24 hours ambient or older than 48 hours refrigerated. Clotted, hemolyzed or frozen specimens. Bone marrow specimens.
- Specimens must be analyzed within 4 days of collection.
New York State Clients: Only the whole blood PNH PAN is acceptable and must be analyzed within 24 hours of collection if ambient or 48 hours of collection if refrigerated.
- Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable
New York State Clients: PNH PAN-Ambient: 24 hours; Refrigerated: 48 hours. Frozen: Unacceptable
The lower limit of detection of this test is 0.005 percent PNH cells and distinguishes between Type II and Type III cells when the PNH cell percentage is 1 percent or greater. Glycophorin A is used to gate the RBC population. CD59 is the GPI-linked antigen. Recent RBC transfusions may decrease the percentage of PNH cells. (Cytometry 42:223-33, 2000).
For the most accurate measurement of the PNH clone size, the PNH WBC (ARUP test code 2005003) is available to assist with therapeutic decisions in conventional PNH.
For initial diagnosis of PNH and analysis of both RBCs and WBCs, Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (ARUP test code 2005006) is available.
|Component Test Code*||Component Chart Name||LOINC|
|2004367||% PNH RBC||33662-8|
- 59 and Glycophorin A
- PI-Linked Antigens on RBCs