Testosterone Free and Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males
Ordering Recommendation

Laboratory reference method for determining free and total testosterone in men. Total or free testosterone is generally adequate for most evaluations of suspected hypogonadism.

Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry
Sun, Wed-Sat
1-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect between 6-10 a.m.  
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transport 1 mL serum or plasma. (Min: 0.4 mL)  
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 2 months  
Reference Interval
Male Free Testosterone, pg/mL
18 years and older: 47-244 pg/mL

Male Total Testosterone, ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL

Interpretive Data

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
84402; 84403
Component Test Code*Component Chart NameLOINC
2003247Testos Free Adult Male ED/LC-MS/MS2991-8
2004245Testosterone Total, LC-MS/MS, Males2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Direct dialysis (Testosterone Free & Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males)
  • Total and Free (Testosterone Free & Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males)