Antiphospholipid Syndrome Reflexive Panel
Ordering Recommendation

Preferred initial panel for strong suspicion of antiphospholipid syndrome (APS).

Electromagnetic Mechanical Clot Detection/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Light blue (sodium citrate) for Lupus Anticoagulant Reflexive Panel (refer to the Specimen Handling at for hemostasis/thrombosis specimens handling guidelines) AND serum separator tube for cardiolipin and beta-2 glycoprotein antibodies.  
Specimen Preparation
Transport 2 mL platelet poor plasma. (Min: 2 mL) AND 1 mL serum (Min: 0.6 mL) in two separate ARUP Standard Transport Tubes.  
Storage/Transport Temperature
Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Serum: Refrigerated  
Unacceptable Conditions
For Lupus Anticoagulant Reflexive Panel (Plasma): Serum. EDTA plasma, clotted or hemolyzed specimens.
For cardiolipin and beta-2 glycoprotein antibodies (Serum):
Plasma and other body fluids, heat-inactivated, hemolyzed, lipemic, or contaminated specimens.  
For Lupus Anticoagulant Reflexive Panel (Plasma): Ambient: 4 hours; Refrigerated: Unacceptable; Frozen: 1 week
For cardiolipin and beta-2 glycoprotein antibodies (Serum):
Ambient: 48 hours; Refrigerated: 2 weeks; Frozen 1 year (avoid repeated freeze/thaw cycles  
Reference Interval
Available Separately
Reference Interval
NoBeta-2 Glycoprotein 1 Antibody, IgGEffective February 18, 2014
0-20 SGU
NoBeta-2 Glycoprotein 1 Antibody, IgMEffective February 18, 2014
0-20 SMU
Yes (0050901)Cardiolipin Antibody, IgGEffective August 18, 2014
0-14 GPL: Negative
15-19 GPL: Indeterminate
20-80 GPL: Low to Moderately Positive
81 GPL or above: High Positive
Yes (0050902)Cardiolipin Antibody, IgMEffective August 18, 2014
0-12 MPL: Negative
13-19 MPL: Indeterminate
20-80 MPL: Low to Moderately Positive
81 MPL or above: High Positive
Yes (0030215)Prothrombin TimeEffective February 18, 2014
12.0-15.5 seconds
Yes (0030235)Partial Thromboplastin TimeEffective February 18, 2014
32-48 seconds
NoDilute Russell Viper Venom Time (dRVVT)Effective February 18, 2014
33-44 seconds
NoThrombin TimeEffective February 18, 2014
14.7-19.5 seconds
NoReptilase TimeEffective February 18, 2014
Less than 22.0 seconds
NoPTT Heparin NeutralizedEffective February 18, 2014
32-48 seconds
NoPartial Thromboplastin Time 1:1 Mix (performed if PTT >48 seconds)Effective February 18, 2014
32-48 seconds
NoPlatelet Neutralization Procedure (performed if PTT 1:1 Mix >48 seconds)Effective February 18, 2014
NoDilute Russell Viper Venom (dRVVT) 1:1 Mix (performed if dRVVT >44 seconds)Effective February 18, 2014
33-44 seconds
NoDilute Russell Viper Venom Time (dRVVT) Confirmation Test (performed if dRVVT 1:1 Mix >44 seconds)Effective February 18, 2014
NoHexagonal Phospholipid NeutralizationEffective February 18, 2014

Interpretive Data
See individual components.

If PTT and dRVVT are normal, then no further testing is performed. If PTT is abnormal, Thrombin Time is added. If Thrombin Time is normal, PTT 1:1 mix is added. If Thrombin time is abnormal, Reptilase Time and PTT Heparin Neutralization are added. If PTT Heparin Neutralization is abnormal, PTT 1:1 mix is added. If PTT 1:1 mix is abnormal, Platelet Neutralization procedure is added. If dRVVT is abnormal, dRVVT 1:1 mix is added. If dRVVT 1:1 mix is abnormal, dRVVT confirmation is added. If Platelet Neutralization procedure and dRVVT confirmation are normal or if one is normal and the other not done, Hexagonal Phospholipid Neutralization is added. Additional charges apply.
CPT Code(s)
86147 x2; 86146 x2; 85610; 85730; 85613; if reflexed, additional CPT codes may apply: 85670; 85635; 85730; 85525; 85732; 85597; 85613 x2; 85598.
Component Test Code*Component Chart NameLOINC
0030079Lupus Anticoagulant Interpretation3281-3
0030082Hexagonal Phospholipid Neutral Reflex33930-9
0030084PTT-D Heparin Neutralized52122-9
0030184Platelet Neutralization (PTT-D, Confirm)3284-7
0030189dRVVT Confirmation52756-4
0030232PTT-LA Screen (PTT-D)34571-0
0030246Prothrombin Time5902-2
0030270Thrombin Time3243-3
0030296Reptilase Time6683-7
0030323PTT-D 1:1 Mix5946-9
0030352dRVVT Screen6303-2
0030353dRVVT 1:1 Mix43397-9
0050322B2Glycoprotein 1, IgG Antibody44448-9
0050323B2Glycoprotein 1, IgM Antibody44449-7
0050901Cardiolipin Antibody IgG3181-5
0050902Cardiolipin Antibody IgM3182-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • ACA
  • aCL antibody
  • Anti-Phospholipid Syndrome Reflexive Panel (Antiphospholipid Syndrome Reflexive Panel)
  • APS panel
  • APS Reflexive Panel
  • B2Glycoprotein I
  • B2GPI
  • Cardiolipin Antibody
  • dRVVT Screen
  • Hexagonal Phospholipid Neutral Reflex
  • Hughes Syndrome Panel
  • Hypercoag
  • Hypercoagulable
  • Lupus Anticoagulant (LA)
  • Platelet Neutralization
  • Prothrombin Time
  • PTT-D Heparin Neutralized
  • Reptilase Time
  • Thrombin Time