Methylphenidate and Metabolite, Urine, Quantitative
Ordering Recommendation
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Mon, Thu 
1-7 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Random urine.  
Specimen Preparation
Transfer 2 mL urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Room temperature specimens.  
Ambient: Unacceptable; Refrigerated: 3 weeks; Frozen: 3 months  
Reference Interval
Effective August 17, 2015
Drugs Covered Methylphenidate
Methylphenidate 10 ng/mL
Ritalinic acid 100 ng/mL
Interpretive Data
Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Positive Cutoff:
Methylphenidate: 10 ng/mL
Ritalinic acid: 100 ng/mL

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
80360; (Alt code: 80299)
Component Test Code*Component Chart NameLOINC
2003116Methylphenidate, Urn, Quant3809-1
2003117Ritalinic acid, Urn, Quant33507-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Attenade
  • Attenade
  • Daytrana
  • Dexmethylphenidate
  • Focalin (Methylphenidate and Metabolite-Confirmation/Quantitation-Urine)
  • Metadate
  • Methylin
  • Methylphenidate
  • Ritalin
  • Ritalinic Acid
  • Urine ritalin concentration