Kappa/Lambda Light Chain Panel by in situ Hybridization, Paraffin
Ordering Recommendation

Quantify kappa and lambda light chains in paraffin. Includes interpretation.

In situ Hybridization
2-5 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Specimen Preparation
Formalin fix (10% neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 10 unstained 5 micron slides in a tissue transport kit (ARUP supply #47808). Available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787. (Min: 5 slides) Protect paraffin block and/or slides from excessive heat.  
Storage/Transport Temperature
Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Specimens fixed or processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Frozen specimens.  
Include surgical pathology report.  
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Available SeparatelyTest NameReference Interval
NoKappa by in situ Hybridization, ParaffinBy report
NoLambda by in situ Hybridization, ParaffinBy report
NoKappa/Lambda Ratio0.5-5.0

Interpretive Data
Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
CPT Code(s)
88368; 88369
Component Test Code*Component Chart NameLOINC
2002887Kappa/Lambda ISH Comment
2002999ISH Tissue Source31208-2
2003000ISH Reference Number30896-5
2003001H and E Slide Description19772-3
2003218Kappa/Lambda ISH Clonality
2003219Kappa/Lambda ISH Ratio
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • K/L ISH
  • Kappa and Lambda ISH
  • Kappa and Lambda mRNA Probes