HLA-DQB Genotyping
2002810
Ordering Recommendation
Do not use for HLA-related disease screening or diagnosis (eg, celiac disease, narcolepsy, rheumatologic diseases). To rule out celiac disease (when other testing is inconclusive), refer to Celiac Disease (HLA-DQA1*05, HLA-DQB1*02, and HLA-DQB1*03:02) Genotyping (2005018). When narcolepsy is suspected in symptomatic individuals, refer to Narcolepsy (HLA-DQB1*06:02) Genotyping (2005023).
Submit With Order
Mnemonic
HLA-DQ DNA
Methodology
Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization
Performed
Mon-Fri
Reported
3-7 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 3 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Specimens collected in green (sodium or lithium heparin).  
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
By report
Interpretive Data
Background Information for HLA-DQB Genotyping:
Purpose:
For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-DQB locus.
Methodology: PCR followed by
Sequence Specific Oligonucleotide Probe Hybridization of HLA-DQB locus.
Analytical Sensitivity & Specificity:
Medium to high resolution of HLA-DQB locus.
Limitations:
The presence of a disease-associated HLA combination does not establish a diagnosis. If less than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation (refer to HLA Bone Marrow Transplantation Evaluation - 2002806).

Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.
Note
Order this test for single antigen HLA-DQB identification. Please specify antigens. Refer to HLA-DR Genotyping for single antigen HLA-DR identification.
CPT Code(s)
81382
Components
Component Test Code*Component Chart Name
2002811HLA Class II, Locus DQB*, Allele 1
2002812HLA Class II, Locus DQB*, Allele 2
2002813HLA-DQB Genotyping Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References