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HLA-C Genotyping (INACTIVE as of 07/02/12: Refer to 2006988)
2002807
Ordering Recommendation
Mnemonic
HLA-C DNA
Methodology
Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization
Performed
Mon-Fri
Reported
5-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). 
Specimen Preparation
Transport 5 mL whole blood. (Min: 3 mL) 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Specimens collected in green (sodium or lithium heparin). 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Background Information for HLA-C Genotyping:
Purpose:
For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-C locus.
Methodology: PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-C locus.
Analytical Sensitivity & Specificity: Low to medium resolution of the HLA-C locus.
Limitations: The presence of a disease-associated HLA combination does not establish a diagnosis. If less than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation (refer to HLA Bone Marrow Transplantation Evaluation-2002806).

Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Note
Order this test for single antigen HLA-C identification. Please specify antigens.
CPT Code(s)
83891 Isolation; 83900 Multiplex Amplification; 83896 x10 Nucleic acid probes; 88384 Probes 11-50; 88385 Probes 51-250; 83912 Interpretation and report -Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.
Components
Component Test Code*Component Chart NameLOINC
0095716HLA C by SSP Interp21026-0
2002808HLA Class I, Locus Cw*, Allele 142663-5
2002809HLA Class I, Locus Cw*, Allele 242663-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases

Performed at UUHC - H&I Lab