- Patient Preparation
- Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).
- Specimen Preparation
- Transport 5 mL whole blood. (Min: 3 mL)
- Storage/Transport Temperature
- Room temperature.
- Unacceptable Conditions
- Specimens collected in green (sodium or lithium heparin).
- Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Purpose: For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-A and B loci.
Methodology: PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-A and HLA-B loci.
Analytical Sensitivity & Specificity: Low to medium resolution of HLA-A and B loci.
Limitations: The presence of a disease-associated HLA combination does not establish a diagnosis. If less than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation (refer to HLA Bone Marrow Transplantation Evaluation-2002806).
Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.
|Component Test Code*||Component Chart Name||LOINC|
|2002785||HLA Class I, Locus B*, Allele 1||13299-3|
|2002786||HLA Class I, Locus B*, Allele 2||13299-3|
|2002790||HLA Class I, Locus A*, Allele 1||38548-4|
|2002791||HLA Class I, Locus A*, Allele 2||38548-4|
|2002848||HLA-AB Genotyping Interpretation|
Performed at UUHC - H&I Lab