Tramadol and Metabolites - Confirmation/Quantitation - Serum or Plasma
Ordering Recommendation
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Tue, Thu
1-6 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles.  
After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years  
Reference Interval
Interpretive Data
Interpretive Data:Methodology: LC-MS/MS

Drugs covered:
Tramadol, n-desmethyltramadol (qualitative only), and o-desmethyltramadol (qualitative only).

Positive cutoff:

Tramadol: 50 ng/mL
n-desmethyltramadol: 100 ng/mL
o-desmethyltramadol: 100 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug may indicate use of parent drug during the prior week.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

See Compliance Statement B:
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
80373; (Alt code: 80299)
Component Test Code*Component Chart NameLOINC
2002741Tramadol, Serum or Plasma 
2002765N-desmethyltramadol, Serum or Plasma 
2002768O-desmethyltramadol, Serum or Plasma 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
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