Tramadol and Metabolites - Confirmation/Quantitation - Urine
Ordering Recommendation
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Tue, Thu
1-6 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Random urine.  
Specimen Preparation
Transfer 2 mL urine with no additives or preservatives an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Room temperature.  
Unacceptable Conditions
Specimens exposed to repeated freeze/thaw cycles.  
Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years  
Reference Interval
Interpretive Data
Methodology: LC-MS/MS

Drugs covered:
Tramadol, n-desmethyltramadol (qualitative only), and o-desmethyltramadol (qualitative only).

Positive cutoff:
Tramadol: 50 ng/mL
n-desmethyltramadol: 100 ng/mL
o-desmethyltramadol: 100 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug is common and may indicate use of parent drug during the prior week.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

See Compliance Statement B:
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
80373; (Alt code: 80299)
Component Test Code*Component Chart NameLOINC
2002737N-desmethyltramadol, Urine 
2002740O-desmethyltramadol, Urine18338-4
2002769Tramadol, Urine17719-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
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