Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae)
Ordering Recommendation
Secondary screening for SLE based on ANA results.
Semi-Quantitative Indirect Fluorescent Antibody
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, or severely lipemic specimens.  
After separation from cells: Ambient 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Less than 1:10  
Interpretive Data
Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). The presence of the anti-dsDNA IgG antibody is identified by IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). CLIFT is highly specific for SLE with a sensitivity of 50-60 percent.

Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the CLIFT result is negative but the patient has a positive ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA.Additional information and recommendations for testing may be found at  
Double Stranded DNA (dsDNA) antibodies (1:10 or greater) are found in 50-60 percent of systemic lupus erythematosus (SLE), 20-30 percent in Sjögren syndrome, 20-25 percent in mixed connective tissue disease (MCTD), and less than 5 percent in progressive systemic sclerosis (PSS). High titers of antibody to native (double stranded) DNA are specific for SLE.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2002692Double-Stranded DNA (dsDNA) Ab IgG IFA58466-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • anti-DNA
  • Antibody to ds-DNA
  • Double stranded DNA antibody
  • Native double-stranded DNA antibody