Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae)
Ordering Recommendation
Secondary screening for SLE based on ANA results.
Submit With Order
Semi-Quantitative Indirect Fluorescent Antibody
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, or severely lipemic specimens.  
After separation from cells: Ambient 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Less than 1:10
Interpretive Data
Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). The presence of the anti-dsDNA IgG antibody is identified by IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). CLIFT is highly specific for SLE with a sensitivity of 50-60 percent.

Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the CLIFT result is negative but the patient has a positive ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA. Additional information and recommendations for testing may be found at
Double Stranded DNA (dsDNA) antibodies (1:10 or greater) are found in 50-60 percent of systemic lupus erythematosus (SLE), 20-30 percent in Sjögren syndrome, 20-25 percent in mixed connective tissue disease (MCTD), and less than 5 percent in progressive systemic sclerosis (PSS). High titers of antibody to native (double stranded) DNA are specific for SLE.
CPT Code(s)
Component Test Code*Component Chart Name
2002692Double-Stranded DNA (dsDNA) Ab IgG IFA
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Cross References
  • anti-DNA
  • Antibody to ds-DNA
  • Double stranded DNA antibody
  • Native double-stranded DNA antibody