Human Immunodeficiency Virus 1 (HIV-1) by Quantitative PCR with Reflex to HIV-1 Genotype by Sequencing
Ordering Recommendation

Detect and quantify HIV-1. Determine HIV drug resistance by DNA sequencing.

Quantitative Polymerase Chain Reaction/Sequencing
2-13 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), pink (K2EDTA), or plasma preparation tube. Multiple tubes may be required. 
Specimen Preparation
Separate plasma from cells within 24 hours. Transfer two 3 mL aliquots of plasma to individual ARUP Standard Transport Tubes. (Min: 3 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Serum. Heparinized specimens. Specimens submitted in a plasma preparation tube. 
Ambient: On cells: 24 hours. After separation from cells: Ambient: 24 hours; Refrigerated: 6 days; Frozen: 6 weeks 
Reference Interval
Available Separately
Reference Interval
0055598Human Immunodeficiency Virus 1 by Quantitative PCRNot detected
0055670Human Immunodeficiency Virus 1, Genotype by SequencingBy report

Interpretive Data
Refer to individual components.

The limit of quantification for the Human Immunodeficiency Virus 1 by Quantitative PCR assay is 1.3 log copies/mL (20 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.3 log copies/mL (<20 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

If Human Immunodeficiency Virus 1 by Quantitative PCR result is greater than or equal to 3.0 log copies/mL, then HIV-1 Genotype by Sequencing will be added. Additional charges apply.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0020297HIV-1 Qnt by PCR (log copy/mL)49890-7
0051817HIV-1 Qnt by PCR Interp24013-5
2002646HIV-1 Qnt by PCR (copy/mL)25836-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • HIV genotyping for drug sensitivity testing
  • HIV-1 reflex to genotyping
  • HIV-1 Treatment decision
  • HIV-1 viral load
  • Nucleic Acid Amplification Test (NAAT)
  • Viral load monitoring