Human Immunodeficiency Virus 1 RNA Quantitative Real-Time PCR with Reflex to Genotype
Ordering Recommendation
Submit With Order
Quantitative Real-Time Polymerase Chain Reaction/Sequencing
2-13 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Lavender (EDTA), pink (K2EDTA), or plasma preparation tube. Multiple tubes may be required.  
Specimen Preparation
Separate plasma from cells within 24 hours. Transfer two 3 mL aliquots of plasma to individual ARUP Standard Transport Tubes. (Min: 3 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Serum. Heparinized specimens. Specimens submitted in a plasma preparation tube.  
Ambient: On cells: 24 hours. After separation from cells: Ambient: 24 hours; Refrigerated: 6 days; Frozen: 6 weeks  
Reference Interval
Available Separately Components Reference Interval
0055598Human Immunodeficiency Virus 1 RNA Quantitative Real-​Time PCR Not detected
0055670Human Immunodeficiency Virus 1, Genotype by Sequencing By report
Interpretive Data
Refer to individual components.
If HIV-1 RNA Quantitative Real-Time PCR is greater than or equal to 3.0 log copies/mL, then HIV-1 Genotyping will be added. Additional charges apply.

The limit of quantification for the HIV-1 RNA Quantitative assay is 1.3 log copies/mL (20 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.3 log copies/mL (<20 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."
CPT Code(s)
87536; if reflexed, add 87901
Component Test Code*Component Chart Name
0020297HIV-1 RNA Qnt Real-Time PCR, Log
0051817HIV-1 RNA Qnt Real-Time PCR Interp
2002646HIV-1 RNA Qnt Real-Time PCR, Copy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • HIV genotyping for drug sensitivity testing
  • HIV-1 reflex to genotyping
  • HIV-1 Treatment decision
  • HIV-1 viral load
  • Nucleic Acid Amplification Test (NAAT)
  • Viral load monitoring