Hepatitis C Virus RNA Quantitative Real-Time PCR with Reflex to Genotype
2002685
Ordering Recommendation
Preferred reflex test to confirm active hepatitis C virus (HCV) infection following positive HCV screen. Reflex to genotype aids in determining therapy of choice, likelihood of response, and probable therapeutic duration. Order only after positive HCV screen.
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Mnemonic
HCV QT GR
Methodology
Quantitative Real-Time Polymerase Chain Reaction/Sequencing
Performed
Sun-Sat
Reported
6-11 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), plasma preparation tube (PPT), or serum separator tube.  
Specimen Preparation
Separate serum or plasma from cells within 6 hours. Transfer 3.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.8 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Heparinized specimens.  
Remarks
 
Stability
On Cells: Ambient: 6 hours; After separation from cells: Refrigerated: 72 hours; Frozen: 6 weeks  
Reference Interval
Available Separately Components Reference Interval
0098268Hepatitis C Virus RNA Quantitative, Real-​Time PCR Not Detected
0055593Hepatitis C Virus Genotype by Sequencing By report
Interpretive Data
Refer to individual components.
Note
The limit of quantification for the HCV Quantitative RNA assay is 1.6 log IU/mL (43 IU/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.6 log IU/mL (<43 IU/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies the test result will be reported as "Not Quantified."

If Hepatitis C Virus RNA Quantitative Real-Time PCR result is greater than or equal to 2.8 log IU/mL, then Hepatitis C Virus Genotyping by PCR & Sequencing will be added. Additional charges apply.
CPT Code(s)
87522; if reflexed, add 87902
Components
Component Test Code*Component Chart Name
0051819HCV RNA Qnt Real-Time PCR Interp
0099030HCV RNA Qnt Real-Time PCR
2002324HCV RNA Qnt Real-Time PCR
2005764EER Hepatitis C RNA Qnt w/Genotype Reflx
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • HCV confirmation
  • HCV genotype
  • HCV quantitative
  • HCV treatment decision
  • HCV-PCR
  • HCV-RNA
  • Hepatitis C Viral Load, Genotype reflex
  • Nucleic Acid Amplification Test (NAAT)
  • Viral load monitoring