BRAF Codon 600 Mutation Detection by Pyrosequencing
Ordering Recommendation
Evaluate suspected Lynch syndrome. Use to predict effectiveness of therapies targeting the EGFR pathway. Evaluate thyroid specimens (tissue blocks or formalin-fixed, paraffin-embedded cell blocks prepared from fine needle aspirates [FNA]) for BRAF mutations for purpose of diagnosis, prognosis, and possible therapeutics. For endometrial tissue evaluation, refer to MLH1 Promoter Methylation, Paraffin (2002499). For FNA direct smears, the preferred test is BRAF V600E Mutation Detection by Allele-Specific PCR, Fine Needle Aspirate (2006516).
Polymerase Chain Reaction/Pyrosequencing
DNA isolation: Sun-Sat
Mon, Wed, Fri
10-14 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides). Tissue block will be returned after testing.
Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives. Decalcified specimens.  
Include surgical pathology report.  
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
88381; 81210
Component Test Code*Component Chart NameLOINC
2002148Block ID57723-9
2002524BRAF Codon 600 Mutation Detection58483-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • BRAF Gene Mutation Assay
  • BRAF Mutation Analysis
  • EGFR targeted therapy
  • sporadic Lynch Syndrome
  • V600E