Congenital Adrenal Hyperplasia Panel, 21-Hydroxylase Deficiency
Ordering Recommendation

Screen for 21-hydroxylase deficiency, the most common congenital adrenal insufficiency.

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect between 6-10 a.m. 
Serum separator tube or green (sodium or lithium heparin). Also acceptable: Pink (K2EDTA). 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1.8 mL serum or plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.9 mL) 
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. 
Unacceptable Conditions
Refrigerated or room temperature specimens. 
After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months 
Reference Interval
Available Separately
Reference Interval
2001638AndrostenedioneEffective August 19, 2013
Premature Infants, 26-28 weeks-Day 40.92-2.82 ng/mL0.92-2.82 ng/mL
Premature Infants, 31-35 weeks-Day 40.80-4.46 ng/mL0.80-4.46 ng/mL
Full-term Infants, 1-7 days0.20-2.90 ng/mL0.20-2.90 ng/mL
8-30 days0.18-0.80 ng/mL0.18-0.80 ng/mL
1-5 months0.06-0.68 ng/mL0.06-0.68 ng/mL
6-24 monthsLess than 0.15 ng/mL0.03-0.15 ng/mL
2-3 yearsLess than 0.16 ng/mLLess than 0.11 ng/mL
4-5 years0.02-0.21 ng/mL0.02-0.17 ng/mL
6-7 years0.02-0.28 ng/mL0.01-0.29 ng/mL
8-9 years0.04-0.42 ng/mL0.03-0.30 ng/mL
10-11 years0.09-1.23 ng/mL0.07-0.39 ng/mL
12-13 years0.24-1.73 ng/mL0.10-0.64 ng/mL
14-15 years0.39-2.00 ng/mL0.18-0.94 ng/mL
16-17 years0.35-2.12 ng/mL0.30-1.13 ng/mL
18-39 years0.26-2.14 ng/mL0.33-1.34 ng/mL
40 years and older0.13-0.82 ng/mL0.23-0.89 ng/mL
Pre-menopausal0.26-2.14 ng/mLDoes Not Apply
Postmenopausal0.13-0.82 ng/mLDoes Not Apply
Tanner Stage I0.05-0.51 ng/mL0.04-0.32 ng/mL
Tanner Stage II0.15-1.37 ng/mL0.08-0.48 ng/mL
Tanner Stage III0.37-2.24 ng/mL0.14-0.87 ng/mL
Tanner Stage IV-V0.35-2.05 ng/mL0.27-1.07 ng/mL

009233217-Hydroxyprogesterone Quantitative by HPLC-MS/MS, Serum or PlasmaEffective August 19, 2013
Premature (26-28 weeks)124-841 ng/dL124-841 ng/dL
Premature (29-35 weeks)26-568 ng/dL26-568 ng/dL
Full term Day 37-77 ng/dL7-77 ng/dL
4 days-30 days7-106 ng/dLLess than 200 ng/dL
1 month-2 months13-106 ng/dLLess than 200 ng/dL
3 months-5 months13-106 ng/dL3-90 ng/dL
6  months-1 yearLess than or equal to 148 ng/dLLess than or equal to 148 ng/dL
2-3 yearsLess than or equal to 256 ng/dLLess than or equal to 228 ng/dL
4-6 yearsLess than or equal to 299 ng/dLLess than or equal to 208 ng/dL
7-9 yearsLess than or equal to 71 ng/dLLess than or equal to 63 ng/dL
10-12 yearsLess than or equal to 129 ng/dLLess than or equal to 79 ng/dL
13-15 years9-208 ng/dL9-140 ng/dL
16-17 yearsLess than or equal to 178 ng/dL24-192 ng/dL
18 years and olderLess than 207 ng/dLLess than 139 ng/dL
Follicular15-70 ng/dLDoes Not Apply
Luteal35-290 ng/dLDoes Not Apply
Tanner Stage ILess than or equal to 74 ng/dLLess than or equal to 62 ng/dL
Tanner Stage IILess than or equal to 164 ng/dLLess than or equal to 104 ng/dL
Tanner Stage III13-209 ng/dLLess than or equal to 151 ng/dL
Tanner Stage IV-V7-170 ng/dL20-173 ng/dL

009233317-Hydroxypregnenolone Quantitative by LC-MS/MS, Serum or Plasma
Premature (26-28 weeks)1219-9799 ng/dL1219-9799 ng/dL
Premature (29-36 weeks)346-8911 ng/dL346-8911 ng/dL
Full Term (1-5 months)229-3104 ng/dL229-3104 ng/dL
6-12 monthsless than or equal to 917ng/dLless than or equal to 917ng/dL
13-23 monthsless than or equal to 592 ng/dLless than or equal to 592 ng/dL
2-4 yearsless than or equal to 280 ng/dLless than or equal to 249 ng/dL
5-6 yearsless than or equal to 350 ng/dLless than or equal to 319 ng/dL
7-9 yearsless than or equal to 212 ng/dLless than or equal to 187 ng/dL
10-12 yearsless than or equal to 398 ng/dLless than or equal to 392 ng/dL
13-15 yearsless than or equal to 407 ng/dL35-465 ng/dL
16-17 yearsless than or equal to 423 ng/dL32-478 ng/dL
18 years and olderLess than 226 ng/dLLess than 442 ng/dL
Tanner Stage Iless than or equal to 235 ng/dLless than or equal to 208 ng/dL
Tanner Stage IIless than or equal to 367 ng/dLless than or equal to 355 ng/dL
Tanner Stage IIIless than or equal to 430 ng/dLless than or equal to 450 ng/dL
Tanner Stage IV-Vless than or equal to 412 ng/dL35-478 ng/dL

2001640Dehydroepiandrosterone, Serum or PlasmaEffective August 19, 2013
PrematureLess than 40 ng/mLLess than 40 ng/mL
0-1 dayLess than 11 ng/mLLess than 11 ng/mL
2-6 daysLess than 8.7 ng/mLLess than 8.7 ng/mL
7 days-1 monthLess than 5.8 ng/mLLess than 5.8 ng/mL
1-5 monthsLess than 2.9 ng/mLLess than 2.9 ng/mL
6-24 monthsLess than 1.9 9 ng/mLLess than 2.5 ng/mL
2-3 yearsLess than 0.85 ng/mLLess than 0.63 ng/mL
4-5 yearsLess than 1.03 ng/mLLess than 0.95 ng/mL
6-7 yearsLess than 1.79 ng/mL0.06-1.93 ng/mL
8-9 years0.14-2.35 ng/mL0.10-2.08 ng/mL
10-11 years0.43-3.78 ng/mL0.32-3.08 ng/mL
12-13 years0.89-6.21 ng/mL0.57-4.10 ng/mL
14-15 years1.22-7.01 ng/mL0.93-6.04 ng/mL
16-17 years1.42-9.00 ng/mL1.17-6.52 ng/mL
18-39 years1.33-7.78 ng/mL1.33-7.78 ng/mL
40 years and older0.63-4.70 ng/mL0.63-4.70 ng/mL
Postmenopausal0.60-5.73 ng/mLDoes Not Apply
Tanner Stage I0.14-2.76 ng/mL0.11-2.37 ng/mL
Tanner Stage II0.83-4.87 ng/mL0.37-3.66 ng/mL
Tanner Stage III1.08-7.56 ng/mL0.75-5.24 ng/mL
Tanner Stage IV-V1.24-7.88 ng/mL1.22-6.73 ng/mL

Interpretive Data

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
009233217-Hydroxyprogesterone, HPLC-MS/MS1668-3
009233317-Hydroxypregnenolone Quant, MS/MS, Ser6765-2
2001639Androstenedione by TMS1854-9
2001641Dehydroepiandrosterone by TMS2193-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 17-Hydroxypregnenolone (CAH 21 Hydroxylase Deficiency Panel)
  • 17-Hydroxyprogesterone (CAH 21 Hydroxylase Deficiency Panel)
  • Androstenedione (CAH 21 Hydroxylase Deficiency Panel)
  • Dehydroepiandrosterone (DHEA) (CAH 21 Hydroxylase Deficiency Panel)