Quantitative Enzyme Immunoassay
New York DOH Approval Status
This test is New York DOH approved.
- Patient Preparation
- Serum separator tube, plain red, lavender (EDTA) or pink (K2EDTA).
- Specimen Preparation
- Allow serum specimen to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Transport 1 mL serum or plasma. (Min: 0.2 mL) Avoid repeated freeze/thaws.)
- Storage/Transport Temperature
- Unacceptable Conditions
- Specimen types other than serum or plasma. Specimens stored or transported at room temperature for more than 48 hours.
- After separation from cells: Ambient: 48 hours; Refrigerated: 10 days; Frozen: 1 year
The ST2 test, an enzyme-linked immunosorbent assay, quantifies soluble ST2 in serum or plasma. ST2 is a member of the interleukin-1 (IL-1) receptor family and is secreted by cardiac muscle cells under conditions of mechanical stress. In individuals with acute myocardial infarction, an increased concentration of soluble ST2 is associated with an increased risk of mortality or heart failure. In individuals with heart failure, an increased concentration of soluble ST2 is strongly associated with the severity of disease and is a strong predictive indicator of 1-year mortality that is independent but additive to that of NT-proBNP.
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