ERBB2 (HER2/neu) (4B5) with Interpretation by Immunohistochemistry, Tissue
2002218
Additional Technical InformationAdditional Technical Information

Immunohistochemistry Stain Form Recommended  (ARUP form #32978)Immunohistochemistry Stain Form Recommended (ARUP form #32978)

Ordering Recommendation
•Aids in prediction of response to trastuzumab (Herceptin) therapy in patients with breast cancer.
•Alternate test to confirm equivocal fluorescence in situ hybridization (FISH) result.
Submit With Order
Mnemonic
4B5 IP
Methodology
Immunohistochemistry
Performed
Mon-Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue within 30 minutes of removal from patient. Fixative duration: 8-48 hours. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect ™ or contact ARUP Client Services at (800)522-2787. (Min: 3 slides) If sending precut slides, do not oven bake.  
Storage/Transport Temperature
Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Paraffin block with no tumor tissue remaining. Specimens with fixation delayed for more than 30 minutes. Specimens fixed in any other fixative other than 10 percent neutral buffered formalin. Tissue fixed for less than 8 hours or greater than 48 hours. Cytology specimens fixed in alcohol.  
Remarks
Pathology report including tissue source and tumor origin must be submitted. Document time from tissue acquisition to fixation and fixation duration on requisition or enter at time of order. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787.  
Stability
Ambient: Indefinite; Refrigerated: Indefinite ; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.
Note
CPT Code(s)
88360
Components
Component Test Code*Component Chart Name
0049171Homogenous Pattern
0049184Intensity of Staining
0049188Percent of Cells/Circumferential
2002219ERBB2(HER2/neu)(4B5) Comments
2002517ERBB2(HER2/neu)(4B5) by IHC
2009596Homogeneous dark circumferential pattern
2009597Uniformity of staining
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • 4B5
  • c-erbB2 (4B5) IHC
  • ERBB2 (4B5) IHC
  • HER-2/neu (4B5) IHC