Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot
2001774
 
Ordering Recommendation
CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117). However, if serology is used to assess late-stage pertussis, this is the recommended test.
Mnemonic
BORDPAN2
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Tue
Reported
1-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Contaminated, heat-inactivated, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
Bordetella pertussis Antibody, IgA by ELISA with Reflex to Immunoblot
Bordetella pertussis Antibody, IgA by ELISA 0.9 U/mL or less: Negative -​ No significant level of detectable Bordetella pertussis IgA antibody.
1.0-​1.1 U/mL: Equivocal -​ Repeat testing in 10-​14 days may be helpful.
1.2 U/mL or greater: Positive -​ IgA antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
Bordetella pertussis Antibody, IgA by Immunoblot Negative
2001768Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
Test Number Components Reference Interval
Bordetella pertussis Antibody IgG by ELISA Effective August 19, 2013
0.0-​0.9 U/mL
2004327Bordetella pertussis Antibody, IgG by Immunoblot Refer to report
Interpretive Data
Refer to report.
Note
If Bordetella pertussis Antibody, IgA by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 1.0 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
CPT Code(s)
86615 x2; if reflexed, add 86615 for each Immunoblot
Components
Component Test Code*Component Chart Name
2001770B. pertussis Ab, IgA by ELISA
2001782B. pertussis Ab, IgG by ELISA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • B. pertussis Abs panel reflex
  • Pertussis Antibody
  • Pertussis IgA and IgG Abs reflex to immunoblot