Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot
Ordering Recommendation

CDC-recommended first-line tests for pertussis are PCR (0065080) and/or culture (0060117). However, if serology is used to assess late-stage pertussis (> than 4 weeks), this is the recommended test.

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Tue, Fri
1-5 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number
Reference Interval
 B. pertussis Ab, IgA by ELISA
Bordetella pertussis Antibody, IgA by ELISA0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgA antibody.
1.0-1.1 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 U/mL or greater: Positive - IgA antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
Bordetella pertussis Antibody, IgA by ImmunoblotNegative

2001768Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
Test Number
Reference Interval
Bordetella pertussis Antibody IgG by ELISAEffective August 19, 2013
0.0-0.9 U/mL
2004327Bordetella pertussis Antibody, IgG by ImmunoblotRefer to report

Interpretive Data
Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
If Bordetella pertussis Antibody, IgA by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 1.0 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
CPT Code(s)
86615 x2; if reflexed, add 86615 for each Immunoblot
Component Test Code*Component Chart NameLOINC
2001770B. pertussis Ab, IgA by ELISA42328-5
2001782B. pertussis Ab, IgG by ELISA42330-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • B. pertussis Abs panel reflex
  • Pertussis Antibody
  • Pertussis IgA and IgG Abs reflex to immunoblot