Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot
2001769
Mnemonic
BORDM
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Tue
Reported
1-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
- Patient Preparation
- Collect
- Serum separator tube.
- Specimen Preparation
- Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
- Storage/Transport Temperature
- Refrigerated.
- Unacceptable Conditions
- Contaminated, heat-inactivated, or severely lipemic specimens.
- Remarks
- Stability
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
| Test Number | Components | Reference Interval | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Bordetella pertussis Antibody, IgM by ELISA | Effective February 20, 2018 0.9 IV or less: Negative - No significant level of detectable B. pertussis IgM antibody. 1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful. 1.2 IV or greater: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis. | |||||||||||||
| 2004326 | Bordetella pertussis Antibody, IgM by Immunoblot |
| ||||||||||||
Interpretive Data
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
Note
If Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
Hotline History
View Hotline History
CPT Code(s)
86615; if reflexed, add 86615
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2001783 | B. pertussis Ab, IgM by ELISA | 42329-3 |
Aliases
- B. pertussis IgM reflex
- Pertussis Antibody
- Pertussis IgM Ab ELISA reflex to immunoblot