Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot
2001769
 
Ordering Recommendation
Evaluation of IgM pertussis antibodies has little clinical utility. CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Mnemonic
BORDM
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Tue
Reported
1-8 days
New York DOH Approval Status
This test will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
Storage/Transport Temperature
Refrigerated.
Unacceptable Conditions
Contaminated, heat-inactivated, or severely lipemic specimens.
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
Test Number Components Reference Interval
Bordetella pertussis Antibody, IgM by ELISA 0.9 U/mL or less: Negative-​No significant level of detectable Bordetellapertussis IgM antibody.
1.0-​1.1 U/mL: Equivocal-​Repeat testing in 10-​14 days may be helpful.
1.2 U/mL or greater: Positive-​IgM antibody to Bordetellapertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004326Bordetella pertussis Antibody, IgM by Immunoblot
Bordetella pertussis Antibody, IgM by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Ab, IgM by Immunoblot Interp Negative
B. pertussis, IgM Immunoblot PT Negative
B. pertussis, IgM Immunoblot FHA Negative
Interpretive Data
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.  
Note
If Bordetella pertussis Antibody, IgM by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
CPT Code(s)
86615 Bordetella IgM; if reflexed add 86615 for Bordetella IgM Immunoblot
Components
Component Test Code*Component Chart Name
2001783B. pertussis Ab, IgM by ELISA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Pertussis Antibody (Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot)
  • Whooping Cough (Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot)