Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
Ordering Recommendation

May be used as evidence of vaccination or past infection; test does not determine immunity to B. pertussis. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117). In most cases, serology testing is not recommended for the diagnosis of active pertussis infection.

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Tue, Fri
1-5 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." 
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. 
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Reference Interval
Bordetella pertussis Antibody IgG by ELISAEffective February 20, 2018
0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004327Bordetella pertussis Antibody, IgG by Immunoblot
Bordetella pertussis Antibody, IgG by Immunoblot
Effective February 19, 2013
Reference Interval
Bordetella pertussis Ab, IgG by Immunoblot InterpNegative
B. pertussis, IgG Immunoblot PT100Negative
B. pertussis, IgG Immunoblot PTNegative
B. pertussis, IgG Immunoblot FHANegative

Interpretive Data
Refer to report.

Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
Hotline History
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CPT Code(s)
Component Test Code*Component Chart NameLOINC
2001782B. pertussis Ab, IgG by ELISA42330-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • B. pertussis IgG reflex
  • Pertussis Antibody
  • Pertussis IgG reflex to immunoblot