Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
2001768
 
Ordering Recommendation
In most cases, serology testing is not recommended for the diagnosis of active pertussis infection. CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Mnemonic
BORDG
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Tue
Reported
1-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
Bordetella pertussis Antibody IgG by ELISA Effective August 19, 2013
0.0-​0.9 U/mL
2004327Bordetella pertussis Antibody, IgG by Immunoblot
Bordetella pertussis Antibody, IgG by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
B. pertussis, IgG Immunoblot PT100 Negative
B. pertussis, IgG Immunoblot PT Negative
B. pertussis, IgG Immunoblot FHA Negative
Interpretive Data
Refer to report.
Note
If Bordetella pertussis Antibody, IgG by ELISA is 1.0 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
CPT Code(s)
86615 Bordetella IgG; if reflexed add 86615 Bordetella IgG Immunoblot
Components
Component Test Code*Component Chart Name
2001782B. pertussis Ab, IgG by ELISA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • B. pertussis IgG reflex
  • Pertussis Antibody
  • Pertussis IgG reflex to immunoblot