QuantiFERON-TB Gold In-Tube and Mycobacterium tuberculosis Antibody, IgG
2001627
 
Ordering Recommendation
Mnemonic
TB PAN
Methodology
Cell Culture/Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Thu
Reported
1-8 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
At altitudes from sea level to 2,650 feet: QuantiFERON-TB (Standard) Gold In-Tube collection kit.
At altitudes from 2,650-3,350 feet:
QuantiFERON-TB (Standard) Gold In-Tube collection kit.
At altitudes from 3,350-6,150 feet:
QuantiFERON-TB (High Altitude) Gold In-Tube collection kit.
At altitudes above 6,150 feet:
QuantiFERON-TB (High Altitude) Gold In-Tube collection kit.  
Specimen Preparation
At altitudes from sea level to 2,650 feet: Whole blood directly into each of the three tubes in the QuantiFERON-TB (Standard) Gold In-Tube collection kit (ARUP supply
#45112) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes from 2,650-3,350 feet:
Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in
QuantiFERON-TB (Standard) Gold In-Tube collection kit (ARUP supply #45112).
At altitudes from 3,350-6,150 feet:
Whole blood directly into each of the three tubes in the QuantiFERON-TB (High Altitude) Gold In-Tube collection
kit (ARUP supply #46261) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes above 6,150 feet:
Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in
QuantiFERON-TB (High Altitude) Gold In-Tube collection kit (ARUP supply #46261).
Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood filling.
Immediately after filling tubes, shake them ten (10) times just firmly enough to ensure the entire inner surface of the tube is coated with blood, to
solubilize antigens on tube walls. Over-energetic shaking may cause gel disruption and could lead to aberrant results.
After mixing,
incubate upright at 37°C for 16-24 hours (within 16 hours of collection).
After incubation,
centrifuge for 15 minutes at 2000-3000 RCF (g).
After centrifugation,
transport entire contents in each of the 3 original collection tubes.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Specimens that have not been incubated and centrifuged.  
Remarks
Further information on mixing QuantiFERON-TB Gold In-Tube collection tubes and an instructional video can be found at: http://www.aruplab.com/Specimen-Handling/SpecialSpecimenCollection/quantiferon.jsp  
Stability
After incubation and centrifugation: Ambient: 2 hours; Refrigerated: 4 weeks; Frozen: 4 months  
Reference Interval
Test Number Components Reference Interval
0051729QuantiFERON TB Gold In-​Tube
QuantiFERON TB Gold In-​Tube
Effective November 19, 2012
Components Reference Interval
QuantiFERON-​TB Gold In-​Tube Negative
QuantiFERON-​TB minus NIL 0.34 IU/mL or less
QuantiFERON MITOGEN minus NIL No reference interval
QuantiFERON NIL No reference interval
0051698Mycobacterium tuberculosis Antibody, IgG by ELISA 0.424 OD or less: Negative -​ No significant level of detectable IgG antibody to Mycobacterium tuberculosis.

0.425-​0.500 OD: Equivocal -​ Repeat testing in 10-​14 days may be helpful.

0.501 OD or greater: Positive -​ IgG antibody to Mycobacterium tuberculosis detected, which may indicate acute or active infection.
Interpretive Data
Refer to individual components for further interpretive data.

See Compliance Statement D: www.aruplab.com/CS
Note
Refer to individual components for further notes.
CPT Code(s)
86480; 86609
Components
Component Test Code*Component Chart Name
0051699M. tuberculosis Antibody, IgG
0051728QuantiFERON- TB Gold In-Tube
2006961QuantiFERON- TB minus NIL
2006962QuantiFERON Mitogen minus NIL
2006963QuantiFERON NIL
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • LTBI QFT-TB IgG
  • QFT & M TB IgG
  • QFT-G & IgG M. tuberculosis
  • Quantiferon Mitogen and NIL