- Patient Preparation
- Lavender (EDTA) or pink (K2EDTA).
- Specimen Preparation
- Transport 5 mL whole blood (Min: 1 mL). Extended exposure to EDTA anticoagulants can result in altered parasite morphology. For best results, send five (5) thin blood smears (unstained, unfixed) AND five (5) thick smears (unstained, unfixed) in addition to whole blood. Thin and thick blood smears should be prepared immediately or within 1 hour after collection.
Thin smears are made as per routine hematology differentials. Thick smears are made by dropping 10 to 20 µL of blood onto a slide and spreading it into a dime-sized area with a glass, wooden, or plastic applicator.
For instructions on preparation of thick and thin smears, see http://www.dpd.cdc.gov/dpdx/HTML/DiagnosticProcedures.htm. Label and transport slides in slide holders. Specimen must be received within 24 hours of collection.
Remarks: Rapid diagnosis of malaria is critical for appropriate patient management. Specimens for Malaria and Babesia are for confirmation only. Travel history required.
- Storage/Transport Temperature
- Room temperature.
- Unacceptable Conditions
- Whole blood in anticoagulants other than EDTA or K2EDTA.
- Rapid diagnosis of malaria is critical for appropriate patient management. Specimens for Malaria and Babesia are for confirmation only from clients outside of the immediate Salt Lake City, Utah area. Travel history required.
- Whole blood: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.
Slides: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable
|Component Test Code*||Component Chart Name||LOINC|
|0049025||Parasites Smear (Giemsa Stain), Blood||51714-4|
|2001548||Malaria, Rapid Screen||46094-9|
- Giemsa Stain for Malaria
- P falciparum HRP2 antigen detection