Cytology, SurePath Liquid-Based Pap Test with Reflex to Human Papillomavirus (HPV), High Risk by PCR, SurePath
Ordering Recommendation

For HPV testing, only FDA-cleared sample preservative (ie, ThinPrep) is recommended. Refer to corresponding ThinPrep testing (2000138).

Microscopy/ Qualitative Polymerase Chain Reaction
1-14 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
1. For premenopausal patients, obtain specimens during the second half of the menstrual period to avoid contamination by obscuring blood.
2. Instruct the patient not to douche or engage in sexual intercourse within 24 hours of the procedure 
1. Label the vial(s) with the patient's first and last name, date of birth, and specimen source before beginning the procedure.
2. Obtain all specimens prior to bimanual examination.
3. Place the patient in the lithotomy position. Using an unlubricated vaginal speculum (saline may be used as a lubricant) visualize the cervix as fully as possible.
4. Follow the instructions for the specific collection kit used. 
Specimen Preparation
Supplies: This test requires a SurePath collection kit, which includes a preservative fluid collection vial and sampling device(s). Kits may be ordered separately through ARUP Client Services at (800) 522-2787 or (801) 583-2787. Also required: ARUP Cytology test request form, permanent marker.

Collection Procedure: Obtain cervical specimen prior to bimanual examination. Use an unlubricated speculum (saline or warm water may be used). Vaginal discharge or secretion, when present in large amounts, should be removed before obtaining the cervical sample so as not to disturb the epithelium (i.e., cellulose swab). Small amounts of blood will not interfere with the cytologic evaluation; however, large amounts of blood as present during menses may interfere with cytologic evaluation because cells may be obscured by blood. Use of liquid-based specimen collection minimizes the interference from these factors. If testing for sexually transmitted disease is indicated, the cervical cytology sample should be taken first. After visualization of the cervix is accomplished, collect the sample. Obtain an adequate sampling from the cervix using the Rovers Cervex-Brush or Cervex-Brush Combi or the Pap Perfect plastic spatula with the Cytobrush Plus GT.

ROVERS CERVEX-BRUSH Collection (Kit #22216)
: Device may be referred to as broom. Position the tip of the longer bristles into the cervical os/endocervical canal. Begin rotating in a clockwise direction (one-quarter to one-half turn). The bristles will begin to stiffen. Continue rotating in a clockwise direction and gently push towards the cervix until the shorter bristles begin to bend extending over the ectocervix. Complete five 360-degree rotations. Direction must be consistent. Do not alter or vary the direction of the broom during sampling. Transfer the entire sample by placing your thumb against the back of the brush pad, and simply disconnect the entire brush from the stem into the preservative vial. Contraindication: Do not use on pregnant patients after the first 10 weeks of gestation.

Insert the contoured end of the Pap Perfect plastic spatula and rotate 360 degrees around the entire exocervix. Disconnect the spatula head and place it in into the vial. Insert the Cytobrush Plus GT into the endocervix until only the bottom most fibers are exposed at the cervical os. Slowly rotate or turn one half turn in one direction. To reduce unnecessary bleeding, DO NOT over rotate. Disconnect the brush head and place in the preservative vial. Contraindication: Do not use the Cytobrush Plus GT (endocervical brush) on pregnant patients or for endometrial sampling.

ROVERS CERVEX-BRUSH COMBI Collection (Kit #45031):
Insert central bristles into the endocervical canal. Use gentle pressure on the cervix until the lateral bristles bend against the ectocervix. Maintain gentle pressure and rotate two times in a clockwise direction by rolling the stem between the thumb and forefinger. Disconnect the brush head and place in the preservative vial. Contraindication: This device should not be used during pregnancy.

For all kits, recap the preservative vial and tighten. Record the patient's information and medical history on the ARUP Cytology test request form. Place the vial and test request form in a specimen bag for transport to the laboratory. 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Specimens not collected in a SurePath collection kit. Expired preservative vials or vials received without the collection devices. 
Ambient: 2 weeks; Refrigerated: 4 weeks; Frozen: Unacceptable 
Reference Interval
Interpretive Data

If the SurePath Liquid-Based Pap Test is interpreted as atypical squamous cells of undetermined significance (ASC-US), then Human Papillomavirus (HPV) High Risk by PCR, SurePath will be added. Additional charges apply.
CPT Code(s)
88142 or 88143, if reviewed by pathologist add 88141.  If reflexed add 87624.
Component Test Code*Component Chart NameLOINC
2000507SurePath Pap Reflex Specimen Source
2000508SurePath Pap Reflex Clinical History
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Pap Test
  • SurePath Pap reflex to HPV HR
  • SurePath Pap Test
  • SurePath Pap Test withreflex to HR HPV