Sirolimus by Tandem Mass Spectrometry
0098467
Ordering Recommendation
Submit With Order
Mnemonic
RAPAMUNE
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Pre-dose (trough) levels should be drawn.  
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 1 mL whole blood. (Min: 0.25 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.  
Remarks
 
Stability
Ambient: 24 hours; Refrigerated: 1 week; Frozen: 2 months  
Reference Interval
Effective February 18, 2014
Therapeutic Range:
Kidney transplant (in combination with Cyclosporine): 4-​12 ng/mL
Toxic value: Greater than 25 ng/mL
Interpretive Data
A range of 12-20 ng/mL has been suggested for liver transplant. The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose, type of transplanted organ, and/or the therapeutic approach of the transplant center.

See Compliance Statement B: www.aruplab.com/CS
Note
Sirolimus (Rapamune) whole blood concentrations can be measured by either chromatographic or immunoassay methodologies. These two methodologies are not directly interchangeable and the measured sirolimus whole blood concentration depends on the methodology used. Reference ranges may vary according to the specific immunoassay or HPLC-MS/MS test. Generally, immunoassays have been reported to have a positive bias relative to HPLC-MS/MS assays due to the detection of antibody cross-reactivity with sirolimus metabolites.
CPT Code(s)
80195
Components
Component Test Code*Component Chart Name
0098467Sirolimus by HPLC-MS/MS
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Rapamune
  • Rapamycin