Hepatitis C Virus by Quantitative PCR
0098268
Ordering Recommendation
Preferred single confirmation test for HCV positive screen. Order only after positive HCV screen. Use to monitor chronic disease.
Mnemonic
HEPCQNT
Methodology
Quantitative Polymerase Chain Reaction
Performed
Sun-Sat
Reported
1-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA), pink (K2EDTA), plasma preparation tube (PPT), or serum separator tube.  
Specimen Preparation
Separate serum or plasma from cells within 24 hours. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Heparinized specimens.  
Remarks
  
Stability
Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: 6 weeks  
Reference Interval
Not Detected  
Interpretive Data
The quantitative range of this assay is 1.2-8.0 log IU/mL (15-100,000,000 IU/mL).

Limit of detection (LOD):
15 IU/mL (1.2 log IU/mL)
LOD values do not apply to diluted specimens.

An interpretation of "Not Detected" does not rule out the presence of PCR inhibitors in the patient specimen or hepatitis C virus RNA concentrations below the level of detection of the test. Care should be taken when interpreting any single viral load determination.

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).
 
Note
The limit of quantification for this RNA assay is 1.2 log IU/mL (15 IU/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.2 log IU/mL (<15 IU/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

Specimens received with less than minimum volume for testing will automatically be run with a dilution according to the guidelines below:
-Specimens with 325-650 µL will be diluted resulting in a modification of the quantitative range of the assay to 1.5-8.3 log IU/mL (30-200,000,000 IU/mL).
-Specimens with 130-325 µL will be diluted resulting in a modification of the quantitative range of the assay to 1.9-8.7 log IU/mL (75-500,000,000 IU/mL).

This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used in assessing viral response to antiretroviral treatment as measured by changes in HCV RNA levels.
CPT Code(s)
87522
Components
Component Test Code*Component Chart NameLOINC
0051819HCV Qnt by PCR Interp11259-9
0099030HCV Qnt by PCR (log IU/mL)38180-6
2002324HCV Qnt by PCR (IU/mL)11011-4
2005724EER HCV Qnt by PCR11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HCV monitoring
  • HCV quantitative
  • HCV Screen confirmation
  • HCV Viral Load
  • Nucleic Acid Amplification Test (NAAT)
  • Viral load monitoring