Cortisol Urine Free by LC-MS/MS
0097222
 
Ordering Recommendation
Mnemonic
CORT UF
Methodology
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
24-hour or random urine. Refrigerate 24-hour specimen during collection.  
Specimen Preparation
Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Room temperature specimens. Acidified specimens or specimens with preservatives.  
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: 2 weeks; Frozen: 6 months  
Reference Interval
Effective April 7, 2014
Test Name Female Male
Cortisol, Urine Free -​ per 24h 3-​8 years: Less than or equal to 18 µg/24h
9-​12 years: Less than or equal to 37 µg/24h
13-​17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 45 µg/24h
3-​8 years: Less than or equal to 18 µg/24h
9-​12 years: Less than or equal to 37 µg/24h
13-​17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 60 µg/24h
Cortisol, Urine Free -​ ratio to CRT Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 24 µg/g CRT
Pregnancy: Less than 59 µg/g CRT
Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 32 µg/g CRT
Interpretive Data
Interpretive Data: The optimal specimen for this testing is a 24-hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with duration of greater than 28 hours, or a collection with total volume less than 400 mL. Ratios to creatinine may be useful for these evaluations.

Baseline urinary free cortisol excretion less than 5 ug/d may be consistent with adrenal insufficiency.

See Compliance Statement B: www.aruplab.com/CS
Note
Reference intervals based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-1519.
CPT Code(s)
82530
Components
Component Test Code*Component Chart Name
0020207Creatinine, Urine - per volume
0020208Creatinine, Urine - per 24h
0092087Cortisol, Urine Free - per volume
0093444Cortisol, Urine Free - ratio to CRT
0097110Total Volume
0097111Hours Collected
0097224Cortisol, Urine Free - per 24h
2006309Cortisol, Urine Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Cortisol, Free, LC/MS/MS, Second Void Urine
  • Urinary Free Cortisol