Lymphocyte Antigen Proliferation  
Ordering Recommendation

Do not order for patients younger than 3 months unless clinical history of candidiasis is present.

Cell Culture
9-10 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient. 
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance. 
Specimen Preparation
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control). 
Storage/Transport Temperature
Unacceptable Conditions
Yellow (ACD Solution B).  Refrigerated or frozen specimens. Specimens in transport longer than 48 hours. 
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation.

Results are reported as the counts per minute (CPM) antigen stimulated versus control cultures and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
Hotline History
Component Test Code*Component Chart NameLOINC
0096055Lymphocyte Ag Proliferation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Antigen Proliferation, Lymphocytes