Lymphocyte Transplantation CD3
0095949
 
Time SensitiveTime Sensitive
Ordering Recommendation
Mnemonic
CD3
Methodology
Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Draw specimen before administering immunosuppressive medications.  
Collect
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.  
Unacceptable Conditions
Clotted, hemolyzed, frozen, or refrigerated specimens. Specimens older than 48 hours.
New York State Clients:
Clotted, hemolyzed, frozen, or refrigerated specimens. EDTA specimens older than 30 hours and heparin specimens older than 48 hours.  
Remarks
THIS TEST IS FOR TRANSPLANT PATIENTS ONLY. WBC and lymph percent results with the same draw date and time as the CD3 specimen must be provided by the client. Specimens must be analyzed within 48 hours of collection. Some medications may affect immunophenotyping results and should be listed on the patient test request form.
New York State Clients:
EDTA specimens must be analyzed within 30 hours of collection. Heparin specimens must be analyzed within 48 hours of collection.  
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
Ambient: 30 hours in EDTA, 48 hours in heparin; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
Effective November 18, 2013
Reports include age appropriate reference intervals and interpretation.
Components 0-​11 months 12-​23 months 2 years and older
WBC (K/µL) Must be provided by the client Must be provided by the client Must be provided by the client
% Lymph Must be provided by the client Must be provided by the client Must be provided by the client
Absolute Lymph Count Calculated from above information Calculated from above information Calculated from above information
%CD3 58-​85 % 53-​81 % 59-​86 %
Abs CD3 2170-​6500 cells/µL 1460-​5440 cells/µL 650-​2500 cells/µL
Interpretive Data
In this test, the percent CD3 cells are those cells that are positive for both CD3 and the alpha-beta chain of the T-cell receptor and are presumed immunologically competent.

See Compliance Statement A: www.aruplab.com/CS
Note
THIS TEST IS FOR TRANSPLANT PATIENTS ONLY. Effective immunosuppressive therapy with OKT3 can be documented by verifying CD3 antigen removal using this test. For immunocompromised patients, order Lymphocyte Subset Panel 4 - T-Cell subsets Percent & Absolute (0095950).
CPT Code(s)
86356 x2
Components
Component Test Code*Component Chart Name
0095248% CD3+/A-B+
0095249Absolute CD3+/A-B+
0095883Absolute Lymph
0097135White Blood Cell Count
0097140% Lymph
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • T-Cell Transplant Monitoring (Lymphocyte Transplantation CD3)