B-Cell Immunodeficiency Profile
0095940
Time SensitiveTime Sensitive

Ordering Recommendation
Useful in supporting the diagnosis of primary B-cell immunodeficiency disorders, including the bare lymphocyte syndrome and agammaglobulinemia.
Submit With Order
Mnemonic
B-CELL
Methodology
Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 3 mL)  
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.  
Unacceptable Conditions
Clotted, frozen, or refrigerated specimens. Specimens older than 48 hours.
New York State Clients:
Clotted, frozen, or refrigerated specimens. Specimens in heparin that are more than 48 hours old and specimens in EDTA that are more than 30 hours old.  
Remarks
Specimens must be analyzed within 48 hours of collection.
New York State Clients:
Specimens in heparin must be analyzed within 48 hours of collection; specimens in EDTA must be analyzed within 30 hours of collection.  
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
Ambient: 48 hours in heparin, 30 hours in EDTA; Refrigerated: Unacceptable; Frozen: Unacceptable.  
Reference Interval
Effective November 18, 2013
Reports include age appropriate reference intervals and interpretation
Components Age: 0-​23 months Age: 2 years and older
% CD19 11-​45 % 6-​23 %
% HLA-​DR 11-​45 % 8-​24 %
% Total Ig 4-​32 % 5-​21 %
% IgG 0-​4 % 0-​1 %
% IgM 4-​32 % 5-​21 %
% IgD 4-​32 % 4-​19 %
% IgA 0-​4 % 0-​1 %
Interpretive Data
The B-cell Immunodeficiency Profile measures circulating B-cells (CD19), their surface immunoglobulins (Total Ig, IgG, IgD, IgM, and IgA) and a common HLA class II antigen. The percentage of lymphocytes expressing CD19, various immunoglobulin fractions and HLA-DR may be altered in certain immunologic abnormalities. For example, HLA-DR is absent on B-cells in the bare lymphocyte syndrome (MHC Class II Deficiency).

See Compliance Statement A: www.aruplab.com/CS
Note
CPT Code(s)
86356 x7
Components
Component Test Code*Component Chart Name
0095834% HLA-DR
0095914% CD19
0095936% SIg Total
0095937% SIg IgM
0095938% SIg IgG
0095939% SIg IgD
0095954% SIg IgA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • B-cell immunodeficiency profile
  • HLA-DR, CD19, SIG IgM, IgG, IgD, IgA and Total assessment
  • immune assessment
  • Surface Immunoglobulin Expression on B-cells