- Patient Preparation
- Lavender Hemogard (EDTA), pink Hemogard (K2EDTA), or green Hemogard (sodium or lithium heparin). Hemogard tubes are preferred for laboratory safety.
- Specimen Preparation
- Transport 4 mL whole blood. (Min: 0.5 mL)
- Storage/Transport Temperature
- CRITICAL ROOM TEMPERATURE.
- Unacceptable Conditions
- Frozen or refrigerated specimens. Specimens older than 72 hours in EDTA or 48 hours in heparin. Clotted or hemolyzed specimens.
- Specimens must be analyzed within 72 hours of collection in EDTA or within 48 hours of collection in heparin. Some medications may affect immunophenotyping results and should be listed on the patient test request form.
This test is not approved for New York State Clients.
- Ambient: 72 hours in EDTA, 48 hours in heparin; Refrigerated: Unacceptable; Frozen: Unacceptable
|Components||Age: 0-11 months||Age: 12-23 months||Age: 2 years and older|
|%CD4||38-62 %||31-54 %||32-64 %|
|Absolute CD4||1580-4850 cells/µL||1020-3600 cells/µL||430-1800 cells/µL|
During HIV infection, antiviral therapy is often initiated when the absolute CD4 count drops below 500 cells/µL. When the absolute CD4 count drops below 200 cells/µL, therapeutic prophylaxis against PCP and other opportunistic infections may be initiated. When the absolute CD4 count drops below 100 cells/µL, prophylaxis against Mycobacterium avium complex is recommended.
For longitudinal studies involving serial monitoring, specimen collections should be performed at the same time of day.
|Component Test Code*||Component Chart Name||LOINC|
|0095621||Lymphocyte Subset Panel 2 Information||45265-6|
- CD4 and absoute Count
- Helper T-Cell level
- Immune Status-Flow Cytometry
- P. jiroveci prophylaxis determination